Meiotic sister chromatid cohesion in holocentric sex chromosomes of three heteropteran species is maintained in absence of axial elements

It has been suggested that in species with monocentric chromosomes axial element (AE) components may be responsible for sister chromatid cohesion during meiosis. To test this hypothesis in species with holocentric chromosomes we selected three heteropteran species with different sex-determining mech...

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Bibliographic Details
Published in:Chromosoma 2000-04, Vol.109 (1-2), p.35-43
Main Authors: Suja, J A, del Cerro, A L, Page, J, Rufas, J S, Santos, J L
Format: Article
Language:English
Subjects:
Animals
Cell Division - genetics
Cell Nucleus - ultrastructure
Chromatids - genetics
Chromatids - metabolism
Chromatids - ultrastructure
Chromosome Segregation - genetics
Heteroptera - genetics
Karyotyping
Male
Meiosis - genetics
Sex Chromosomes - genetics
Sex Chromosomes - ultrastructure
Sister Chromatid Exchange - genetics
Sister Chromatid Exchange - physiology
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Summary:It has been suggested that in species with monocentric chromosomes axial element (AE) components may be responsible for sister chromatid cohesion during meiosis. To test this hypothesis in species with holocentric chromosomes we selected three heteropteran species with different sex-determining mechanisms. We observed in surface-spreads and sections using transmission electron microscopy that the univalent sex chromosomes form neither AEs nor synaptonemal complexes (SCs) during pachytene. We also found that a polyclonal antibody recognizing SCP3/Cor1, a protein present at AEs and SC lateral elements of rodents, labels the autosomal SCs but not AEs or SC stretches corresponding to the sex chromosomes. Cytological analysis of the segregational behaviour of the sex univalents demonstrates that although these chromosomes segregate equationally during anaphase I they never show precocious separation of sister chromatids during late prophase I or metaphase I. These results suggest that AEs are not responsible for sister cohesion in sex chromosomes. The segregational behaviour of these chromosomes during both meiotic divisions also indicates that different achiasmate modes of chromosome association exist in heteropteran species.
ISSN:0009-5915
1432-0886
DOI:10.1007/s004120050410