Functional states of polymorphonuclear leukocytes determined by chemiluminescent kinetic analysis

During the respiratory burst, upon stimulation with both soluble and particulate matter, polymorphonuclear leukocytes (PMN) generate reactive oxygen species (ROS) and emit chemiluminescence (CL) as a result of metabolic activation. The measurement of CL has been demonstrated to be a useful tool for...

Full description

Saved in:
Bibliographic Details
Published in:Luminescence (Chichester, England) England), 2000-05, Vol.15 (3), p.143-151
Main Authors: Magrisso, Moni Y., Alexandrova, Margarita L., Markova, Vanja I., Bechev, Blagovest G., Bochev, Petyo G.
Format: Article
Language:English
Subjects:
Adult
blood
Case-Control Studies
component model
functional states
Humans
In Vitro Techniques
Infection - blood
Kinetics
Luminescent Measurements
Neutrophils - metabolism
polymorphonuclear leukocytes
reactive oxygen species
Reactive Oxygen Species - metabolism
Respiratory Burst
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:During the respiratory burst, upon stimulation with both soluble and particulate matter, polymorphonuclear leukocytes (PMN) generate reactive oxygen species (ROS) and emit chemiluminescence (CL) as a result of metabolic activation. The measurement of CL has been demonstrated to be a useful tool for in vitro assessment of the opsonophagocytic function of PMN. Using component analysis of CL kinetics, we characterized the functional state of PMN by three parameters of the respiratory burst: capacity, effectiveness and velocity (CEV space). The possibility of delimiting eight different functional states of PMN is discussed. The CL kinetics shown by blood PMN in different functional states was analysed, and revealed six out of eight functional states. We conclude that CEV‐estimated functional states of PMN are relative, depending on both PMN readiness to generate ROS and conditions of the CL test. © 2000 John Wiley & Sons, Ltd.
ISSN:1522-7235
1522-7243
DOI:10.1002/1522-7243(200005/06)15:3<143::AID-BIO577>3.0.CO;2-R