A Plant Viral “Reinitiation” Factor Interacts with the Host Translational Machinery

The cauliflower mosaic virus transactivator, TAV, controls translation reinitiation of major open reading frames on polycistronic RNA. We show here that TAV function depends on its association with polysomes and eukaryotic initiation factor eIF3 in vitro and in vivo. TAV physically interacts with eI...

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Bibliographic Details
Published in:Cell 2001-09, Vol.106 (6), p.723-733
Main Authors: Park, Hyun-Sook, Himmelbach, Axel, Browning, Karen S., Hohn, Thomas, Ryabova, Lyubov A.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Brassica - genetics
Brassica - virology
Cauliflower mosaic virus
Caulimovirus - genetics
Caulimovirus - physiology
Cloning, Molecular
Conserved Sequence
elF3g protein
Eukaryotic Initiation Factor-3
Gene Expression Regulation, Plant
Genes, Plant
initiation factor eIF3
L24 protein
Molecular Sequence Data
Open Reading Frames
Peptide Initiation Factors - metabolism
Polyribosomes - metabolism
Protein Biosynthesis
Ribosomal Proteins - metabolism
Ribosomes - metabolism
RNA, Messenger - genetics
Saccharomyces cerevisiae - genetics
Sequence Alignment
Sequence Homology, Amino Acid
TAV protein
Trans-Activators - chemistry
Trans-Activators - genetics
Trans-Activators - metabolism
Transcription, Genetic
Triticum - genetics
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Summary:The cauliflower mosaic virus transactivator, TAV, controls translation reinitiation of major open reading frames on polycistronic RNA. We show here that TAV function depends on its association with polysomes and eukaryotic initiation factor eIF3 in vitro and in vivo. TAV physically interacts with eIF3 and the 60S ribosomal subunit. Two proteins mediating these interactions were identified: eIF3g and 60S ribosomal protein L24. Transient expression of eIF3g and L24 in plant protoplasts strongly affects TAV-mediated reinitiation activity. We demonstrate that TAV/eIF3/40S and eIF3/TAV/60S ternary complexes form in vitro, and propose that TAV mediates efficient recruitment of eIF3 to polysomes, allowing translation of polycistronic mRNAs by reinitiation, overcoming the normal cell barriers to this process.
ISSN:0092-8674
1097-4172
DOI:10.1016/S0092-8674(01)00487-1