Pharmaceutical-Induced Cell Apoptosis Characterized by Capillary Zone Electrophoresis

Capillary zone electrophoresis (CZE) with a non-gel-sieving system was employed to characterize actinomycin D-induced cell apoptosis by measuring cellular DNA damage, termed DNA ladder, which proved to be thoroughly different from the DNA damage pattern of cell necrosis. The results by CZE analyses...

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Bibliographic Details
Published in:Analytical biochemistry 2001-10, Vol.297 (1), p.10-14
Main Authors: Liu, Bi-Feng, Zhang, Lei, Liu, Hai-Hua, Lu, Ying-Tang
Format: Article
Language:English
Subjects:
Animals
apoptosis
Apoptosis - drug effects
capillary electrophoresis
Cell Line
Dactinomycin - pharmacology
DNA damage
DNA Damage - drug effects
DNA Fragmentation - drug effects
DNA ladder
Electrophoresis, Agar Gel
Electrophoresis, Capillary - methods
fluorescence detection
Insecta - cytology
Necrosis
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Summary:Capillary zone electrophoresis (CZE) with a non-gel-sieving system was employed to characterize actinomycin D-induced cell apoptosis by measuring cellular DNA damage, termed DNA ladder, which proved to be thoroughly different from the DNA damage pattern of cell necrosis. The results by CZE analyses were identical to those obtained by conventional slab gel electrophoresis, demonstrating that CZE would be a reliable and more convenient technique for the identification of cell death with advantages of higher performance, high-speed, minute sample requirement, and advanced automation.
ISSN:0003-2697
1096-0309
DOI:10.1006/abio.2001.5305