Use of osmotically active agents to alter optical properties of tissue: Effects on the detected fluorescence signal measured through skin

Background and Objective Hyper‐osmotic chemical agents were used to study the effects of transient tissue scattering on the remitted fluorescence emission intensity from a target placed under a tissue sample. Study Design/Materials and Methods A fluorescent film was placed underneath in vitro and in...

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Bibliographic Details
Published in:Lasers in surgery and medicine 2001-09, Vol.29 (3), p.213-220
Main Authors: Vargas, Gracie, Chan, Kin F., Thomsen, Sharon L., Welch, A.J.
Format: Article
Language:English
Subjects:
Analysis of Variance
Animals
Biological and medical sciences
controlling optical properties
Cricetinae
Dimethyl Sulfoxide - pharmacokinetics
DMSO
Fluorescence
glucose
Glucose - pharmacokinetics
glycerol
Glycerol - pharmacokinetics
In Vitro Techniques
index matching
Medical sciences
Mesocricetus
Microscopy
Optics and Photonics
Osmosis - physiology
Rats
Rats, Sprague-Dawley
Scattering, Radiation
Skin - drug effects
Skin - pathology
Skin Physiological Phenomena - drug effects
Skin plastic surgery
Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases
tissue optical clearing
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Summary:Background and Objective Hyper‐osmotic chemical agents were used to study the effects of transient tissue scattering on the remitted fluorescence emission intensity from a target placed under a tissue sample. Study Design/Materials and Methods A fluorescent film was placed underneath in vitro and in vivo samples of hamster skin, and the remitted fluorescent signal traveling to the tissue surface was monitored over time as the tissue was treated with an osmotically active agent. Results The detected fluorescent signal increased as the scattering in tissue samples was substantially reduced. The increase was greater for dimethyl sulfoxide than glucose or glycerol. It was not statistically different between in vivo skin and in vitro skin. Conclusions The study shows how chemical agents can be used to improve the detected signal for a specific optical application. It could be useful in a number of optical therapeutic and diagnostic applications that can benefit from an increase in the penetration depth of light. Lasers Surg. Med. 29:213–220, 2001. © 2001 Wiley‐Liss, Inc.
ISSN:0196-8092
1096-9101
DOI:10.1002/lsm.1110