A Study on the Nitric Oxide Production-Suppressing Activity of Sanguisorbae Radix Components

The active components of an aqueous extract of Sanguisorbae Radix, which possesses nitric oxide (NO) production-suppressing activity, were determined using macrophages that were activated by the addition of lipopolysaccharide. Significant inhibitory activity against the formation of both NO and indu...

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Bibliographic Details
Published in:Biological & pharmaceutical bulletin 2000/06/01, Vol.23(6), pp.717-722
Main Authors: YOKOZAWA, Takako, CHEN, Cui Ping, TANAKA, Takashi, KITANI, Kenichi
Format: Article
Language:English
Subjects:
active component
Animals
Anthocyanins - chemistry
Anthocyanins - pharmacology
Biological and medical sciences
Cells, Cultured
Drugs, Chinese Herbal - chemistry
Drugs, Chinese Herbal - pharmacology
Gallic Acid - analogs & derivatives
Gallic Acid - chemistry
Gallic Acid - pharmacology
General pharmacology
Glucosides - chemistry
Glucosides - pharmacology
Hydrolyzable Tannins
inducible nitric oxide synthase
Magnetic Resonance Spectroscopy
Male
Medical sciences
Mice
Mice, Inbred BALB C
NADPH Dehydrogenase - metabolism
NADPH-diaphorase
nitric oxide
Nitric Oxide - biosynthesis
Nitric Oxide Synthase - metabolism
Nitric Oxide Synthase Type II
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
Proanthocyanidins
sanguiin H-6
Sanguisorbae Radix
Spectrometry, Mass, Fast Atom Bombardment
Tannins - chemistry
Tannins - pharmacology
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Description
Summary:The active components of an aqueous extract of Sanguisorbae Radix, which possesses nitric oxide (NO) production-suppressing activity, were determined using macrophages that were activated by the addition of lipopolysaccharide. Significant inhibitory activity against the formation of both NO and inducible NO synthase, and NADPH-diaphorase activity, which is involved in NO generation, was shown by Sanguisorbae Radix fractions T-B and T-C. On further fractionation, the subfractions of T-B and T-C all showed high anti-NO activity.Sanguiin H-6, sanguiin H-11, 1, 2, 3, 4, 6-penta-O-galloyl-β-D-glucose, eugeniin and polymeric proanthocyanicdin were isolated from TB-3 and TC-4, and all were identified as exhibiting strong anti-NO activity. We have confirmed that sanguiin H-6 is the most active component of Sanguisobae Radix with respect to the suppression of NO production. It is suggested that tannin makes a prominent contribution to the biological activity of Sanguisorbae Radix.
ISSN:0918-6158
1347-5215
DOI:10.1248/bpb.23.717