Determination of hydroperoxides in liposomes by the modified idf and the modified Tiron methods

Two new methods, the modified International Dairy Federation and the modified Tiron methods, were developed for the determination of hydroperoxides in liposomes. Interferences of alpha-tocoferol and egg-phosphatidylcholine (EPC) required a solid-phase extraction before the analysis to eliminate alph...

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Bibliographic Details
Published in:Journal of AOAC International 2000-05, Vol.83 (3), p.589-596
Main Authors: GABRIËLS, M, CIRUNAY, J, ALAFANDY, M, BRISAERT, M, DE TAEVERNIER, M, CAMU, F, PLAIZIER-VERCAMMEN, J
Format: Article
Language:English
Subjects:
1,2-Dihydroxybenzene-3,5-Disulfonic Acid Disodium Salt
Benzene Derivatives - analysis
Biological and medical sciences
Food industries
Fundamental and applied biological sciences. Psychology
Hydrogen Peroxide - analysis
Indicators and Reagents
Liposomes - chemistry
Milk and cheese industries. Ice creams
Nephelometry and Turbidimetry
Peroxides - analysis
Reference Standards
Sensitivity and Specificity
Solutions
Vitamin E
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Summary:Two new methods, the modified International Dairy Federation and the modified Tiron methods, were developed for the determination of hydroperoxides in liposomes. Interferences of alpha-tocoferol and egg-phosphatidylcholine (EPC) required a solid-phase extraction before the analysis to eliminate alpha-tocoferol and a fluid-fluid extraction based on the solvent triangle of Bligh and Dyer to separate EPC. The developed color, using thiocyanate and Tiron, respectively, as complex-formers for the generated ferri-ions, was measured spectrophotometrically. Both techniques showed good reproducibility and high sensitivity. Peroxide contents of 0.04 and 0.07% (g/g) in EPC samples were easily determined.
ISSN:1060-3271
1944-7922
DOI:10.1093/jaoac/83.3.589