Reconstitution of a Functional Core Polycomb Repressive Complex

The opposing actions of polycomb (PcG) and trithorax group (trxG) gene products maintain essential gene expression patterns during Drosophila development. PcG proteins are thought to establish repressive chromatin structures, but the mechanisms by which this occurs are not known. Polycomb repressive...

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Bibliographic Details
Published in:Molecular cell 2001-09, Vol.8 (3), p.545-556
Main Authors: Francis, Nicole J, Saurin, Andrew J, Shao, Zhaohui, Kingston, Robert E
Format: Article
Language:English
Subjects:
Animals
Chromatin - metabolism
chromatin remodeling
DNA - metabolism
DNA Restriction Enzymes
DNA-Binding Proteins - chemistry
DNA-Binding Proteins - isolation & purification
DNA-Binding Proteins - metabolism
Drosophila
Drosophila melanogaster - genetics
Drosophila melanogaster - growth & development
Drosophila melanogaster - metabolism
Drosophila Proteins
Immunoblotting
Insect Proteins - chemistry
Insect Proteins - isolation & purification
Insect Proteins - metabolism
Macromolecular Substances
Nucleoproteins - chemistry
Nucleoproteins - isolation & purification
Nucleoproteins - metabolism
Nucleosomes - metabolism
PcG protein
Polycomb protein
Polycomb Repressive Complex 1
Precipitin Tests
Recombinant Proteins - metabolism
Repressor Proteins - metabolism
Ribonucleoprotein, U1 Small Nuclear - genetics
Ribonucleoprotein, U1 Small Nuclear - metabolism
RNA-Binding Proteins
Templates, Genetic
Transcription Factors - genetics
Transcription Factors - metabolism
Trithorax protein
trxG protein
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Summary:The opposing actions of polycomb (PcG) and trithorax group (trxG) gene products maintain essential gene expression patterns during Drosophila development. PcG proteins are thought to establish repressive chromatin structures, but the mechanisms by which this occurs are not known. Polycomb repressive complex 1 (PRC1) contains several PcG proteins and inhibits chromatin remodeling by trxG-related SWI/SNF complexes. We have defined a functional core of PRC1 by reconstituting a stable complex using four recombinant PcG proteins. One subunit, PSC, can also inhibit chromatin remodeling on its own. These PcG proteins create a chromatin structure that has normal nucleosome organization and is accessible to nucleases but excludes hSWI/SNF.
ISSN:1097-2765
1097-4164
DOI:10.1016/S1097-2765(01)00316-1