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Humoral immune response to cruzipain and cardiac dysfunction in chronic Chagas disease
The humoral immune response to epitopes expressed on cruzipain was evaluated in 31 Chagas disease patients (CDP) with different degrees of cardiac dysfunction. We took advantage of the availability of anti- Trypanosoma cruzi microsomal fraction monoclonal antibodies (MoAbs) reactive with epitopes th...
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| Published in: | Immunology letters 2001-10, Vol.78 (3), p.135-142 |
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| Main Authors: | , , , |
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| cites | cdi_FETCH-LOGICAL-c444t-de75b04b6dd9529cc6873343489275767850803fc67069d27e3ddc90f0067ee63 |
| container_end_page | 142 |
| container_issue | 3 |
| container_start_page | 135 |
| container_title | Immunology letters |
| container_volume | 78 |
| creator | Duschak, Vilma G Riarte, Adelina Segura, Elsa L Laucella, Susana A |
| description | The humoral immune response to epitopes expressed on cruzipain was evaluated in 31 Chagas disease patients (CDP) with different degrees of cardiac dysfunction. We took advantage of the availability of anti-
Trypanosoma cruzi microsomal fraction monoclonal antibodies (MoAbs) reactive with epitopes that are recognized (5A9B11) or not recognized (1A10C11) by CDP sera. The 5A9B11- and 1A10C11-like epitopes are expressed on cruzipain. The reactivity of 5A9B11 against cruzipain was completely inhibited by sera of severe cardiopathy patients while a partial inhibition was found with sera from chagasic patients with mild disease. CDP sera did not block cruzipain recognition by 1A10C11. The antigenic determinants recognized by CDP sera appeared to be linear and carbohydrate free. When the overall anti-cruzipain immune response was evaluated, 70% of CDP with severe disease showed cruzipain titers higher than 1/800 while none of them displayed titers lower that 1/400. This report shows for the first time that the humoral immune response against epitopes expressed on cruzipain appeared to be related with the severity of chronic Chagas disease. |
| doi_str_mv | 10.1016/S0165-2478(01)00255-3 |
| format | article |
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Trypanosoma cruzi microsomal fraction monoclonal antibodies (MoAbs) reactive with epitopes that are recognized (5A9B11) or not recognized (1A10C11) by CDP sera. The 5A9B11- and 1A10C11-like epitopes are expressed on cruzipain. The reactivity of 5A9B11 against cruzipain was completely inhibited by sera of severe cardiopathy patients while a partial inhibition was found with sera from chagasic patients with mild disease. CDP sera did not block cruzipain recognition by 1A10C11. The antigenic determinants recognized by CDP sera appeared to be linear and carbohydrate free. When the overall anti-cruzipain immune response was evaluated, 70% of CDP with severe disease showed cruzipain titers higher than 1/800 while none of them displayed titers lower that 1/400. This report shows for the first time that the humoral immune response against epitopes expressed on cruzipain appeared to be related with the severity of chronic Chagas disease.</description><identifier>ISSN: 0165-2478</identifier><identifier>EISSN: 1879-0542</identifier><identifier>DOI: 10.1016/S0165-2478(01)00255-3</identifier><identifier>PMID: 11578687</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Adult ; Aged ; Antibodies, Blocking - blood ; Antibodies, Protozoan - biosynthesis ; Antibodies, Protozoan - blood ; Antibodies, Protozoan - metabolism ; Antibody Specificity - immunology ; Antigens, Protozoan - chemistry ; Antigens, Protozoan - immunology ; Binding Sites, Antibody ; Binding, Competitive - immunology ; Carbohydrates - blood ; Carbohydrates - immunology ; Chagas Cardiomyopathy - blood ; Chagas Cardiomyopathy - immunology ; Chagas Cardiomyopathy - physiopathology ; Chagas disease ; Chronic Disease ; Cruzipain ; Cysteine Endopeptidases - immunology ; Enzyme-Linked Immunosorbent Assay ; Epitopes - blood ; Epitopes - chemistry ; Epitopes - immunology ; Humans ; Middle Aged ; Protozoan Proteins ; Trypanosoma cruzi</subject><ispartof>Immunology letters, 2001-10, Vol.78 (3), p.135-142</ispartof><rights>2001 Elsevier Science B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c444t-de75b04b6dd9529cc6873343489275767850803fc67069d27e3ddc90f0067ee63</citedby><cites>FETCH-LOGICAL-c444t-de75b04b6dd9529cc6873343489275767850803fc67069d27e3ddc90f0067ee63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11578687$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Duschak, Vilma G</creatorcontrib><creatorcontrib>Riarte, Adelina</creatorcontrib><creatorcontrib>Segura, Elsa L</creatorcontrib><creatorcontrib>Laucella, Susana A</creatorcontrib><title>Humoral immune response to cruzipain and cardiac dysfunction in chronic Chagas disease</title><title>Immunology letters</title><addtitle>Immunol Lett</addtitle><description>The humoral immune response to epitopes expressed on cruzipain was evaluated in 31 Chagas disease patients (CDP) with different degrees of cardiac dysfunction. We took advantage of the availability of anti-
Trypanosoma cruzi microsomal fraction monoclonal antibodies (MoAbs) reactive with epitopes that are recognized (5A9B11) or not recognized (1A10C11) by CDP sera. The 5A9B11- and 1A10C11-like epitopes are expressed on cruzipain. The reactivity of 5A9B11 against cruzipain was completely inhibited by sera of severe cardiopathy patients while a partial inhibition was found with sera from chagasic patients with mild disease. CDP sera did not block cruzipain recognition by 1A10C11. The antigenic determinants recognized by CDP sera appeared to be linear and carbohydrate free. When the overall anti-cruzipain immune response was evaluated, 70% of CDP with severe disease showed cruzipain titers higher than 1/800 while none of them displayed titers lower that 1/400. This report shows for the first time that the humoral immune response against epitopes expressed on cruzipain appeared to be related with the severity of chronic Chagas disease.</description><subject>Adult</subject><subject>Aged</subject><subject>Antibodies, Blocking - blood</subject><subject>Antibodies, Protozoan - biosynthesis</subject><subject>Antibodies, Protozoan - blood</subject><subject>Antibodies, Protozoan - metabolism</subject><subject>Antibody Specificity - immunology</subject><subject>Antigens, Protozoan - chemistry</subject><subject>Antigens, Protozoan - immunology</subject><subject>Binding Sites, Antibody</subject><subject>Binding, Competitive - immunology</subject><subject>Carbohydrates - blood</subject><subject>Carbohydrates - immunology</subject><subject>Chagas Cardiomyopathy - blood</subject><subject>Chagas Cardiomyopathy - immunology</subject><subject>Chagas Cardiomyopathy - physiopathology</subject><subject>Chagas disease</subject><subject>Chronic Disease</subject><subject>Cruzipain</subject><subject>Cysteine Endopeptidases - immunology</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Epitopes - blood</subject><subject>Epitopes - chemistry</subject><subject>Epitopes - immunology</subject><subject>Humans</subject><subject>Middle Aged</subject><subject>Protozoan Proteins</subject><subject>Trypanosoma cruzi</subject><issn>0165-2478</issn><issn>1879-0542</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><recordid>eNqFkMFOGzEQhi3UioS0jwDyqYLDlvGuvfaeEIpaghSJA7RXy7FnwShrB3sXCZ6eDYngyGXmMN_M_PoIOWbwmwGrz2_HIoqSS3UK7AygFKKoDsiUKdkUIHj5jUw_kAk5yvkRgImKV4dkwpiQqlZySv4vhi4ms6a-64aANGHexJCR9pHaNLz6jfGBmuCoNcl5Y6l7ye0QbO9joOPIPqQYvKXzB3NvMnU-o8n4g3xvzTrjz32fkX9__9zNF8Xy5up6frksLOe8LxxKsQK-qp1rRNlYO2aqxohcNaUUspZKgIKqtbWEunGlxMo520ALUEvEupqRX7u7mxSfBsy97ny2uF6bgHHIWrKScSHUlyBToFSpqhEUO9CmmHPCVm-S70x60Qz01rx-N6-3WjUw_W5eb_dO9g-GVYfuc2uvegQudgCOPp49Jp2tx2DR-YS21y76L168AetKkj4</recordid><startdate>20011001</startdate><enddate>20011001</enddate><creator>Duschak, Vilma G</creator><creator>Riarte, Adelina</creator><creator>Segura, Elsa L</creator><creator>Laucella, Susana A</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>20011001</creationdate><title>Humoral immune response to cruzipain and cardiac dysfunction in chronic Chagas disease</title><author>Duschak, Vilma G ; Riarte, Adelina ; Segura, Elsa L ; Laucella, Susana A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c444t-de75b04b6dd9529cc6873343489275767850803fc67069d27e3ddc90f0067ee63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Antibodies, Blocking - blood</topic><topic>Antibodies, Protozoan - biosynthesis</topic><topic>Antibodies, Protozoan - blood</topic><topic>Antibodies, Protozoan - metabolism</topic><topic>Antibody Specificity - immunology</topic><topic>Antigens, Protozoan - chemistry</topic><topic>Antigens, Protozoan - immunology</topic><topic>Binding Sites, Antibody</topic><topic>Binding, Competitive - immunology</topic><topic>Carbohydrates - blood</topic><topic>Carbohydrates - immunology</topic><topic>Chagas Cardiomyopathy - blood</topic><topic>Chagas Cardiomyopathy - immunology</topic><topic>Chagas Cardiomyopathy - physiopathology</topic><topic>Chagas disease</topic><topic>Chronic Disease</topic><topic>Cruzipain</topic><topic>Cysteine Endopeptidases - immunology</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Epitopes - blood</topic><topic>Epitopes - chemistry</topic><topic>Epitopes - immunology</topic><topic>Humans</topic><topic>Middle Aged</topic><topic>Protozoan Proteins</topic><topic>Trypanosoma cruzi</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Duschak, Vilma G</creatorcontrib><creatorcontrib>Riarte, Adelina</creatorcontrib><creatorcontrib>Segura, Elsa L</creatorcontrib><creatorcontrib>Laucella, Susana A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Immunology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Duschak, Vilma G</au><au>Riarte, Adelina</au><au>Segura, Elsa L</au><au>Laucella, Susana A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Humoral immune response to cruzipain and cardiac dysfunction in chronic Chagas disease</atitle><jtitle>Immunology letters</jtitle><addtitle>Immunol Lett</addtitle><date>2001-10-01</date><risdate>2001</risdate><volume>78</volume><issue>3</issue><spage>135</spage><epage>142</epage><pages>135-142</pages><issn>0165-2478</issn><eissn>1879-0542</eissn><abstract>The humoral immune response to epitopes expressed on cruzipain was evaluated in 31 Chagas disease patients (CDP) with different degrees of cardiac dysfunction. We took advantage of the availability of anti-
Trypanosoma cruzi microsomal fraction monoclonal antibodies (MoAbs) reactive with epitopes that are recognized (5A9B11) or not recognized (1A10C11) by CDP sera. The 5A9B11- and 1A10C11-like epitopes are expressed on cruzipain. The reactivity of 5A9B11 against cruzipain was completely inhibited by sera of severe cardiopathy patients while a partial inhibition was found with sera from chagasic patients with mild disease. CDP sera did not block cruzipain recognition by 1A10C11. The antigenic determinants recognized by CDP sera appeared to be linear and carbohydrate free. When the overall anti-cruzipain immune response was evaluated, 70% of CDP with severe disease showed cruzipain titers higher than 1/800 while none of them displayed titers lower that 1/400. This report shows for the first time that the humoral immune response against epitopes expressed on cruzipain appeared to be related with the severity of chronic Chagas disease.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>11578687</pmid><doi>10.1016/S0165-2478(01)00255-3</doi><tpages>8</tpages></addata></record> |
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| ispartof | Immunology letters, 2001-10, Vol.78 (3), p.135-142 |
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| language | eng |
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| source | Elsevier |
| subjects | Adult Aged Antibodies, Blocking - blood Antibodies, Protozoan - biosynthesis Antibodies, Protozoan - blood Antibodies, Protozoan - metabolism Antibody Specificity - immunology Antigens, Protozoan - chemistry Antigens, Protozoan - immunology Binding Sites, Antibody Binding, Competitive - immunology Carbohydrates - blood Carbohydrates - immunology Chagas Cardiomyopathy - blood Chagas Cardiomyopathy - immunology Chagas Cardiomyopathy - physiopathology Chagas disease Chronic Disease Cruzipain Cysteine Endopeptidases - immunology Enzyme-Linked Immunosorbent Assay Epitopes - blood Epitopes - chemistry Epitopes - immunology Humans Middle Aged Protozoan Proteins Trypanosoma cruzi |
| title | Humoral immune response to cruzipain and cardiac dysfunction in chronic Chagas disease |
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