An AraC-like transcriptional activator is required for induction of genes needed for α-galactoside utilization in Sinorhizobium meliloti

The nodulating bacterium Sinorhizobium meliloti can utilize α-galactosides like melibiose and raffinose as sole sources of carbon and energy. We show that this utilization requires an AraC-like transcriptional activator, AgpT. When agpT was inactivated, Rhizobium meliloti could not utilize α-galacto...

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Bibliographic Details
Published in:FEMS microbiology letters 2000-07, Vol.188 (1), p.23-27
Main Authors: Bringhurst, Ryan M, Gage, Daniel J
Format: Article
Language:English
Subjects:
a-Galactosides
AgpT
agpT protein
Alkaline Phosphatase - metabolism
Amino Acid Sequence
AraC
AraC Transcription Factor
Bacterial Proteins
Bacteriology
Biological and medical sciences
Culture Media
Fundamental and applied biological sciences. Psychology
Galactosides - metabolism
Gene Expression Regulation, Bacterial
Genetics
Melibiose
Microbiology
Molecular Sequence Data
Mutation
Phenotype
Raffinose
Repressor Proteins - chemistry
Repressor Proteins - metabolism
Sequence Analysis, DNA
Sinorhizobium meliloti
Sinorhizobium meliloti - genetics
Sinorhizobium meliloti - metabolism
Symbiosis
Trans-Activators - chemistry
Trans-Activators - genetics
Trans-Activators - metabolism
Transcription Factors
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Summary:The nodulating bacterium Sinorhizobium meliloti can utilize α-galactosides like melibiose and raffinose as sole sources of carbon and energy. We show that this utilization requires an AraC-like transcriptional activator, AgpT. When agpT was inactivated, Rhizobium meliloti could not utilize α-galactosides or induce genes required for transport and catabolism of these sugars. The agpT gene was not essential for the establishment of an effective nitrogen-fixing symbiosis.
ISSN:0378-1097
1574-6968
DOI:10.1016/S0378-1097(00)00207-X