A model for the gamma delta resolvase synaptic complex

The serine recombinase gamma delta resolvase performs site-specific recombination in an elaborate synaptic complex containing 12 resolvase subunits and two 114-base pair res sites. Here we present an alternative structural model for the synaptic complex. Resolvase subunits in the complex contact the...

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Bibliographic Details
Published in:Molecular cell 2001-09, Vol.8 (3), p.623-631
Main Authors: Sarkis, G J, Murley, L L, Leschziner, A E, Boocock, M R, Stark, W M, Grindley, N D
Format: Article
Language:English
Subjects:
DNA - metabolism
g^d resolvase
Macromolecular Substances
Models, Molecular
Nucleic Acid Conformation
Protein Conformation
Protein Structure, Quaternary
Protein Structure, Tertiary
Protein Subunits
recombinase
Recombinases
Recombination, Genetic - physiology
synaptic complex
Transposases - chemistry
Transposases - genetics
Transposases - metabolism
Transposon Resolvases
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Summary:The serine recombinase gamma delta resolvase performs site-specific recombination in an elaborate synaptic complex containing 12 resolvase subunits and two 114-base pair res sites. Here we present an alternative structural model for the synaptic complex. Resolvase subunits in the complex contact their neighbors in equivalent ways, using three principal interactions, one of which is a newly proposed synaptic interaction. Evidence in support of this interaction is provided by mutations at the interface that either enable resolvase to synapse two copies of site I or inhibit synapsis of complete res sites. In our model, the two crossover sites are far apart, separated by the resolvase catalytic domains bound to them. Thus, recombination would require a substantial rearrangement of resolvase subunits or domains.
ISSN:1097-2765
DOI:10.1016/S1097-2765(01)00334-3