Phenotypic Characterization of Monocyte Subpopulations in the Pig

We have recently described the existence of two subsets of porcine monocytes based on the expression of CD163.In this study we compare the expression of a number of cell surface antigens in CD169 +and CD163 -monocyte subsets using three-color flow cytometry.These monocyte subsets show differences wi...

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Bibliographic Details
Published in:Immunobiology (1979) 2000-05, Vol.202 (1), p.82-93
Main Authors: Chamorro, Sonia, Revilla, Concepción, Álvarez, Belén, López-Fuertes, Laura, Ezquerra, Ángel, Domínguez, Javier
Format: Article
Language:English
Subjects:
Animals
Antigens, CD - immunology
Cell Differentiation - immunology
histocompatibility antigen SLA
Immunophenotyping
Monocytes - cytology
Monocytes - immunology
Swine - immunology
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Summary:We have recently described the existence of two subsets of porcine monocytes based on the expression of CD163.In this study we compare the expression of a number of cell surface antigens in CD169 +and CD163 -monocyte subsets using three-color flow cytometry.These monocyte subsets show differences with respect to the expression of MHC class II antigens (SLA-DR and DQ) and a variety of adhesion molecules (CDlla, wCD11c, wCD29, CD49d) that are expressed at higher levels on CD163 +monocytes, and of CD14 that is higher expressed by CD163 -cells.These differences on phenotype could reflect differences in the ability of these two subsets to migrate to tissues and may account for the higher allostimulatory capacity of CD163 +cells.In some aspects, the phenoype of CD163 +monocytes resembles that of mature macrophages. In vitroserum-induced maturation of monocytes into macro phages lead to the expression of SWC9 together with an increase in the expression of CD 163 and a reduction in that of CD 14.These results delineate a maturation pathway where CD14 hiCD163 -SWC9 -monocytes develop into CD14 loCD163 +SWC9 -monocytes and these cells into CD14 loCD163 +SWC9 +macrophages.
ISSN:0171-2985
1878-3279
DOI:10.1016/S0171-2985(00)80055-8