The complete nucleotide sequence of the mitochondrial genome of sugar beet (Beta vulgaris L.) reveals a novel gene for tRNA(Cys)(GCA)

We determined the complete nucleotide sequence of the mitochondrial genome of an angiosperm, sugar beet (Beta vulgaris cv TK81-O). The 368 799 bp genome contains 29 protein, five rRNA and 25 tRNA genes, most of which are also shared by the mitochondrial genome of Arabidopsis thaliana, the only other...

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Bibliographic Details
Published in:Nucleic acids research 2000-07, Vol.28 (13), p.2571-2576
Main Authors: Kubo, T, Nishizawa, S, Sugawara, A, Itchoda, N, Estiati, A, Mikami, T
Format: Article
Language:English
Subjects:
Arabidopsis - genetics
Base Sequence
Chenopodiaceae - cytology
Chenopodiaceae - genetics
Conserved Sequence - genetics
DNA, Mitochondrial - chemistry
DNA, Mitochondrial - genetics
Genes, Plant - genetics
Genes, rRNA - genetics
Genome
Introns - genetics
Mitochondria - genetics
Mutation - genetics
Nucleic Acid Conformation
Open Reading Frames - genetics
Physical Chromosome Mapping
Pseudogenes - genetics
Repetitive Sequences, Nucleic Acid - genetics
RNA, Messenger - analysis
RNA, Messenger - genetics
RNA, Transfer, Cys - chemistry
RNA, Transfer, Cys - genetics
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Summary:We determined the complete nucleotide sequence of the mitochondrial genome of an angiosperm, sugar beet (Beta vulgaris cv TK81-O). The 368 799 bp genome contains 29 protein, five rRNA and 25 tRNA genes, most of which are also shared by the mitochondrial genome of Arabidopsis thaliana, the only other completely sequenced angiosperm mitochondrial genome. However, four genes identified here (namely rps13, trnF-GAA, ccb577 and trnC2-GCA) are missing in Arabidopsis mitochondria. In addition, four genes found in Arabidopsis (ccb228, rpl2, rpl16 and trnY2-GUA) are entirely absent in sugar beet or present only in severely truncated form. Introns, duplicated sequences, additional reading frames and inserted foreign sequences (chloroplast, nuclear and plasmid DNA sequences) contribute significantly to the overall size of the sugar beet mitochondrial genome. Nevertheless, 55.6% of the genome has no obvious features of information. We identified a novel tRNA(Cys) gene (trnC2-GCA) which shows no sequence homology with any tRNA(Cys) genes reported so far in higher plants. Intriguingly, this tRNA gene is actually transcribed into a mature tRNA, whereas the native tRNA(Cys) gene (trnC1-GCA) is most likely a pseudogene.
ISSN:1362-4962
DOI:10.1093/nar/28.13.2571