Riluzole stimulates nerve growth factor, brain-derived neurotrophic factor and glial cell line-derived neurotrophic factor synthesis in cultured mouse astrocytes

Riluzole is an antiexcitotoxic agent used for the treatment of amyotrophic lateral sclerosis, and reported to have neuroprotective effects in animal models of Parkinson's disease, Huntington's disease and brain ischemia. We investigated the effects of riluzole on synthesis of nerve growth...

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Bibliographic Details
Published in:Neuroscience letters 2001-09, Vol.310 (2), p.117-120
Main Authors: Mizuta, Ikuko, Ohta, Mitsuhiro, Ohta, Kiyoe, Nishimura, Masataka, Mizuta, Eiji, Kuno, Sadako
Format: Article
Language:English
Subjects:
Animals
Astrocytes
Astrocytes - cytology
Astrocytes - drug effects
Astrocytes - metabolism
Biological and medical sciences
Brain-derived neurotrophic factor
Brain-Derived Neurotrophic Factor - biosynthesis
Cells, Cultured
Glial Cell Line-Derived Neurotrophic Factor
Medical sciences
Mice
Mice, Inbred ICR
Nerve growth factor
Nerve Growth Factor - biosynthesis
Nerve Growth Factors - biosynthesis
Nerve Tissue Proteins - biosynthesis
Neuropharmacology
Neuroprotection
Neuroprotective agent
Neuroprotective Agents - pharmacology
Pharmacology. Drug treatments
Riluzole
Riluzole - pharmacology
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Summary:Riluzole is an antiexcitotoxic agent used for the treatment of amyotrophic lateral sclerosis, and reported to have neuroprotective effects in animal models of Parkinson's disease, Huntington's disease and brain ischemia. We investigated the effects of riluzole on synthesis of nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF) and glial cell line-derived neurotrophic factor (GDNF) in cultured mouse astrocytes. The protein and mRNA levels were measured by enzyme-linked immunosorbent assay and semiquantitative reverse transcription-polymerase chain reaction, respectively. Treatment with riluzole at 100 μg/ml (426 μM) for 24 h increased the contents of NGF, BDNF, and GDNF in the culture medium 109-fold, 2.0-fold and 3.1-fold over the control, respectively. The drug-induced relative mRNA levels of NGF, BDNF, and GDNF were 7.3-fold at 2 h, 2.1-fold at 4 h, and 1.9-fold at 2 h, respectively. These results indicate that riluzole stimulates synthesis of NGF, BDNF and GDNF in cultured astrocytes. Riluzole might exert neuroprotective effects, at least in part, via stimulation of neurotrophic factors.
ISSN:0304-3940
1872-7972
DOI:10.1016/S0304-3940(01)02098-5