Development of a Stable Isotope Approach for the Inductively Coupled Plasma–Mass Spectrometry Determination of Oxidized Metallothionein in Biological Materials

The use of isotope dilution analysis (IDA) with inductively coupled plasma–mass spectrometry (ICP–MS) for the determination of oxidized metallothionein (MT) by a Cd-saturation method is investigated. The method developed here is a modification of an earlier methodology which used a radioactive Cd is...

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Bibliographic Details
Published in:Analytical biochemistry 2000-07, Vol.282 (2), p.194-199
Main Authors: Valles Mota, J.Pablo, Linde Arias, A.Rosa, Fernández de la Campa, M.Rosario, García Alonso, J.Ignacio, Sanz-Medel, Alfredo
Format: Article
Language:English
Subjects:
Acetonitriles
Animals
Cadmium
Horses
Indicator Dilution Techniques
inductively coupled plasma–mass spectrometry
isotope dilution analysis
Isotopes
Kidney
Liver
Mass Spectrometry - methods
metallothionein
Metallothionein - analysis
Oxidation-Reduction
Rats
Rats, Inbred LEC
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Summary:The use of isotope dilution analysis (IDA) with inductively coupled plasma–mass spectrometry (ICP–MS) for the determination of oxidized metallothionein (MT) by a Cd-saturation method is investigated. The method developed here is a modification of an earlier methodology which used a radioactive Cd isotope (109Cd). While retaining the many advantages of this previous approach, the procedure presented here uses stable isotope ratio measurements (114Cd/111Cd) for the determination of MT. Experimental parameters governing the instrumental precision and accuracy for isotope ratio measurements of Cd by ICP–MS were characterized. Systematic errors, including mass bias, detector dead time, and spectroscopic interferences, could be easily corrected. The isotope dilution ICP–MS method was validated by the determination of very low levels of cadmium in biological certified reference materials (NIST SRM 2670 freeze-dried urine, IAEA H-8 horse kidney, and BCR TP-25 lichens). Finally, the IDA procedure was evaluated for the determination of oxidized MT by a Cd-saturation method previously developed using radioactive 109Cd. The final procedure was applied to the quantification of MT in Long–Evans Cinnamon rat liver cytosol samples and the results were compared with data obtained for the same samples using the reference 109Cd methodology. A good agreement between the analytical values obtained by both methods was observed.
ISSN:0003-2697
1096-0309
DOI:10.1006/abio.2000.4607