Molecular Cloning and Characterization of a Novel Human Gene Containing Ankyrin Repeat and Double BTB/POZ Domain

A novel human gene containing an ankyrin repeat and BTB/POZ domains (BPOZ) was isolated from a human leukocyte cDNA library. The cDNA sequence contains an open reading frame of 1434 bp that encodes 478 amino acid residues with a predicted molecular mass of 53.9 kDa. Sequence pattern analysis shows t...

Full description

Saved in:
Bibliographic Details
Published in:Biochemical and biophysical research communications 2000-07, Vol.273 (3), p.991-996
Main Authors: Dai, Ken-Shwo, Wei, Wei, Liew, Choong-Chin
Format: Article
Language:English
Subjects:
Amino Acid Sequence
ankyrin repeat region
Ankyrins - genetics
Base Sequence
BPOZ gene
BTB/POZ domain
chromosomal mapping
chromosome 3
Chromosome Mapping
Chromosomes, Human, Pair 3
Cloning, Molecular
DNA, Complementary
Humans
leukocyte
Molecular Sequence Data
Repetitive Sequences, Amino Acid
Repressor Proteins - genetics
reverse transcription-polymerase chain reaction
Sequence Homology, Amino Acid
Transcription Factors
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A novel human gene containing an ankyrin repeat and BTB/POZ domains (BPOZ) was isolated from a human leukocyte cDNA library. The cDNA sequence contains an open reading frame of 1434 bp that encodes 478 amino acid residues with a predicted molecular mass of 53.9 kDa. Sequence pattern analysis shows that BPOZ contains an N-terminal ankyrin repeat, a bipartite nuclear localization signal and two BTB/POZ domains. Using semiquantitative RT-PCR, the BPOZ transcript was found to be ubiquitously expressed in all fetal tissues examined (heart, brain, liver, and kidney) suggesting that BPOZ is involved in basic cellular function. Low expression of BPOZ in adult tissues (normal and hypertrophic heart) suggests that BPOZ mRNA is developmentally regulated and may play a role in developmental processes. Chromosomal localization by radiation hybrid mapping revealed that this gene is localized between D3S1269 and D3S3606 markers corresponding to the region of chromosome 3q21, a region frequently associated with leukemia. It is thus suggested that BPOZ may be functionally involved in protein-protein interaction, perhaps in forming protein complexes, and may have an important role in normal development and in the development of leukemia.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.2000.3053