Cellular distribution of isozymes of protein kinase C in septal olfactory epithelium of mice

• Published in: Neuroscience letters 2000-07, Vol.288 (2), p.143-146
• Main Authors: Kang, Wha Sun, Lee, Woon Kyu, Seo, Min-Seok, Kim, Jong Kee, Han, Ji Won, Moon, Young Wha, Cho, Kyung-Keun, Kim, Myung Jun, Lee, Kweon-Haeng, Kim, Yong-Sik, Jo, Yang-Hyeok, Choi, Chang Rak
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Blotting, Western; Brain - enzymology; Distribution; Fundamental and applied biological sciences. Psychology; Immunohistochemistry; Isoenzymes - biosynthesis; Isoenzymes - metabolism; Isozyme; Mice; Mice, Inbred ICR; nasal septum; Nasal Septum - enzymology; Neurons, Afferent - enzymology; olfactory glandular cells; Olfactory Mucosa - enzymology; Olfactory system and olfaction. Gustatory system and gustation; Protein kinase C; Protein Kinase C - biosynthesis; Protein Kinase C - metabolism; Sepal olfactory epithelium; Vertebrates: nervous system and sense organs
• ISSN: 0304-3940; 1872-7972
• DOI: 10.1016/S0304-3940(00)01230-1

To determine the presence of protein kinase C (PKC) isozymes in the septal olfactory epithelium of mice (mSOE), western blotting and immunohistochemistry were performed using antibodies against PKC isozymes. With the exception of PKC-βI, all of the PKC isozymes were detected in the whole lysate of septal tissue layer and apparent molecular weights for each isoform were found. PKC-α, PKC-γ and PKC-ε were detected in the olfactory glandular cells of the lamina propria, and PKC-βI and PKC-βII were located in the microvillar cells. Neither novel PKC nor atypical PKC was detected in olfactory glandular cells or microvillar cells, except for PKC-ε. PKC-λ was localized in the mucous layer of the mSOE. Meanwhile, PKC-δ and PKC-ξ were distributed in the receptor cells in the mSOE. These data demonstrate the isoform-specific expression of PKC in mSOE and suggest a role for the novel and atypical types of PKC in olfactory transduction.