A comparison of four serological tests for the diagnosis of caseous lymphadenitis in sheep and goats

A double antibody sandwich ELISA (ELISA A) developed for the detection of Corynebacterium pseudotuberculosis infection in sheep and goats was modified to improve its sensitivity. To establish the sensitivity and specificity of this modified ELISA (ELISA B), sera from 183 sheep and 186 goats were tes...

Full description

Saved in:
Bibliographic Details
Published in:Veterinary microbiology 2000-07, Vol.75 (2), p.167-175
Main Authors: Dercksen, Daan P, Brinkhof, Jan M.A, Dekker-Nooren, Toos, Maanen, Kees van, Bode, Carel F, Baird, Graham, Kamp, Elbarte M
Format: Article
Language:English
Subjects:
Abscess - diagnosis
Abscess - microbiology
Animals
Antibodies, Bacterial - blood
Antibodies, Monoclonal
Bacteriological methods and techniques used in bacteriology
Bacteriology
Biological and medical sciences
CAPRIN
CAPRINOS
Caseous lymphadenitis
Corynebacterium Infections - diagnosis
Corynebacterium Infections - microbiology
Corynebacterium Infections - veterinary
CORYNEBACTERIUM PSEUDOTUBERCULOSIS
Corynebacterium pseudotuberculosis - isolation & purification
DIAGNOSIS
DIAGNOSTIC
DIAGNOSTICO
ELISA
Enzyme-Linked Immunosorbent Assay - veterinary
Fundamental and applied biological sciences. Psychology
Goat Diseases - diagnosis
Goat Diseases - microbiology
GOATS
IMMUNODIAGNOSIS
IMMUNODIAGNOSTIC
INMUNODIAGNOSTICO
Lymphadenitis - diagnosis
Lymphadenitis - microbiology
Lymphadenitis - veterinary
Microbiology
OVIN
OVINOS
Predictive Value of Tests
Sensitivity
Sensitivity and Specificity
SHEEP
Sheep Diseases - diagnosis
Sheep Diseases - microbiology
Sheep-bacteria
Specificity
TEST ELISA
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A double antibody sandwich ELISA (ELISA A) developed for the detection of Corynebacterium pseudotuberculosis infection in sheep and goats was modified to improve its sensitivity. To establish the sensitivity and specificity of this modified ELISA (ELISA B), sera from 183 sheep and 186 goats were tested using ELISAs A and B. Comparison was also made with two further ELISAs (C and D) developed in Australia that, respectively, detect antibodies to cell wall antigens or toxin. ELISA B had the best performance of the four tests. Its specificity was 98±1% for goats and 99±1% sheep. Its sensitivity was 94±3% for goats and 79±5% for sheep. ELISA B will now be tested for use in caseous lymphadenitis eradication and control programmes in The Netherlands. It will also be used in experimental studies of CL in Scotland.
ISSN:0378-1135
1873-2542
DOI:10.1016/S0378-1135(00)00217-0