Establishment of a ciliated epithelial cell line from human Fallopian tube

• Published in: Human reproduction (Oxford) 2000-07, Vol.15 (7), p.1597-1603
• Main Authors: Ando, Hisao, Kobayashi, Miya, Toda, Shigeru, Kikkawa, Fumitaka, Masahashi, Tetsuo, Mizutani, Shigehiko
• Format: Article
• Language: English
• Subjects: Animal cells; Biological and medical sciences; Blastocyst - physiology; Cell cultures. Hybridization. Fusion; Cell Line, Transformed; Cilia - ultrastructure; co-culture; Coculture Techniques; Cytokines - metabolism; Cytological Techniques; Embryo, Mammalian - physiology; Embryonic and Fetal Development; Epithelial Cells - cytology; Epithelial Cells - metabolism; Epithelial Cells - ultrastructure; Fallopian Tubes - cytology; Fallopian Tubes - metabolism; Fallopian Tubes - ultrastructure; Female; Fundamental and applied biological sciences. Psychology; human blastocyst; Humans; in-vitro fertilization; leukaemia inhibitory factor; Liver - cytology; Liver - embryology; Molecular and cellular biology; oviduct
• ISSN: 0268-1161; 1460-2350
• DOI: 10.1093/humrep/15.7.1597

Human tubal epithelial cells in primary culture were transfected with simian virus 40 (SV40) large T antigen plasmid, and an immortalized ciliated cell line, named as NT/T-S, was established without crisis. Transmission electron microscopy proved that NT/T-S cells had cilia, microvilli, junctional complexes, rough endoplasmic reticula, free ribosomes and microtubules. NT/T-S cells were evaluated preliminarily on the basis of co-culture study using surplus embryos at the 4- to 8-cell stage in our IVF and embryo transfer programme. All of the 133 embryos had ≥10% fragments (based on the surface area) and were unworthy of cryopreservation. Up to 57% (16/28) of the embryos with 10–30% fragments reached the blastocyst stage by co-culture. In contrast, blastocyst formation was observed in