Retrograde venous perfusion with hypothermic saline and adenosine for protection of the ischemic spinal cord

• Published in: Journal of vascular surgery 2000-07, Vol.32 (1), p.171-178
• Main Authors: Parrino, Patrick E., Kron, Irving L., Ross, Scott D., Shockey, Kimberly S., Fisher, Michael J., Gaughen, John R., Kallmes, David F., Kern, John A., Tribble, Curtis G.
• Format: Article
• Language: English
• Subjects: Adenosine - therapeutic use; Animals; Aorta; Biological and medical sciences; Constriction; Disease Models, Animal; Female; Hypothermia, Induced - methods; Intraoperative Complications - prevention & control; Ischemia - prevention & control; Male; Medical sciences; Perfusion - methods; Sodium Chloride - therapeutic use; Spinal Cord - blood supply; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Swine; Vascular surgery: aorta, extremities, vena cava. Surgery of the lymphatic vessels; Vasodilator Agents - therapeutic use
• ISSN: 0741-5214; 1097-6809
• DOI: 10.1067/mva.2000.105003

Purpose: Spinal cord injury and the resultant postoperative paraplegia are devastating complications of thoracic aortic surgery, for which no widely accepted protective interventions exist. We hypothesized that retrograde venous perfusion-cooling of the spinal cord with a hypothermic saline and adenosine solution would protect it from ischemic injury caused by thoracic aortic occlusion. Methods: Adult domestic swine of either sex (weight range, 20 to 30 kg) were intubated and ventilated. A left thoracotomy was performed. The accessory hemiazygous vein was divided, and a catheter was inserted distally. The aorta was clamped at the left subclavian artery. The venous catheter was not used in the animals in the control group (n = 7); in the animals in the experimental group (n = 7), a cold (4°C) saline and adenosine solution was infused into the accessory hemiazygous vein. After 30 minutes, the clamp and catheter were removed, and the chest was closed. A blinded observer evaluated the animals' hind-leg motor activity 24 hours later. The Tarlov scale was used: 0, complete paralysis; 1, minimal movement; 2, stands with assistance; 3, stands alone; 4, weak walk; 5, normal gait. The animals' rectal temperatures were measured at the end of the experiment, and blood pressure was measured throughout. Two other groups were studied to assess the effect of the intervention on spinal cord temperature. Results: The animals in the control group had a mean Tarlov score of 1.7 ± 0.6; the animals in the experimental group had a mean Tarlov score of 4.9 ± 0.1 (P