IL-6 PROMOTER IS MODULATED BY THE 24 kDa FGF-2 ISOFORM FUSED TO THE HORMONE BINDING DOMAIN OF THE OESTROGEN RECEPTOR

Fusion proteins consisting of the 24kDa nuclear form of basic fibroblast growth factor (FGF-2), associated with the hormone binding domain of oestrogen receptor (HBD), convey oestrogen inducibility to FGF-2. When stable HBD-FGF-2 HeLa cell lines were transiently transfected with an interleukin 6 (IL...

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Bibliographic Details
Published in:Cytokine (Philadelphia, Pa.) Pa.), 2000-07, Vol.12 (7), p.1110-1114
Main Authors: Delrieu, I, Chinestra, P, Delassus, F, Bayard, F, Prats, H, Faye
Format: Article
Language:English
Subjects:
Binding Sites
Blotting, Western
Down-Regulation
Estradiol - metabolism
Estradiol - pharmacology
Estrogens - metabolism
FGF-2/oestrogen receptor/IL-6
Fibroblast Growth Factor 2 - genetics
Fibroblast Growth Factor 2 - metabolism
Fluorescent Antibody Technique
HeLa Cells
Humans
Interleukin-6 - genetics
Promoter Regions, Genetic
Protein Binding
Protein Isoforms - genetics
Protein Isoforms - metabolism
Receptors, Estrogen - genetics
Receptors, Estrogen - metabolism
Recombinant Fusion Proteins - genetics
Recombinant Fusion Proteins - metabolism
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Summary:Fusion proteins consisting of the 24kDa nuclear form of basic fibroblast growth factor (FGF-2), associated with the hormone binding domain of oestrogen receptor (HBD), convey oestrogen inducibility to FGF-2. When stable HBD-FGF-2 HeLa cell lines were transiently transfected with an interleukin 6 (IL-6) construct, the IL-6 promoter activity was downregulated by the addition of oestradiol. Moreover, in these cell lines, the function of the FGF-2 nuclear localisation sequence was abolished by its fusion to HBD, while addition of oestradiol restored the location of the chimera to the nucleus.
ISSN:1043-4666
1096-0023
DOI:10.1006/cyto.1999.0693