The ISWI Chromatin-Remodeling Protein Is Required for Gene Expression and the Maintenance of Higher Order Chromatin Structure In Vivo

Drosophila ISWI, a highly conserved member of the SWI2/SNF2 family of ATPases, is the catalytic subunit of three chromatin-remodeling complexes: NURF, CHRAC, and ACF. To clarify the biological functions of ISWI, we generated and characterized null and dominant-negative ISWI mutations. We found that...

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Bibliographic Details
Published in:Molecular cell 2000-02, Vol.5 (2), p.355-365
Main Authors: Deuring, Renate, Fanti, Laura, Armstrong, Jennifer A, Sarte, Melinda, Papoulas, Ophelia, Prestel, Matthias, Daubresse, Gary, Verardo, Megan, Moseley, Sarah L, Berloco, Maria, Tsukiyama, Toshio, Wu, Carl, Pimpinelli, Sergio, Tamkun, John W
Format: Article
Language:English
Subjects:
Acetylation
ACF protein
Adenosine Triphosphatases - genetics
Adenosine Triphosphatases - isolation & purification
Adenosine Triphosphatases - metabolism
Animals
Cell Survival
CHRAC protein
Chromatin - ultrastructure
Chromosomes - ultrastructure
DNA-Binding Proteins
Drosophila - anatomy & histology
Drosophila - embryology
Drosophila - genetics
Drosophila melanogaster
Drosophila Proteins
Euchromatin
Female
Fluorescent Antibody Technique
Gene Expression
Genes, Essential
Heterochromatin - ultrastructure
Homeodomain Proteins - isolation & purification
Homeodomain Proteins - metabolism
ISWI protein
Male
Mitosis
Mutation
NURF protein
Phenotype
Transcription Factors - genetics
Transcription Factors - isolation & purification
Transcription Factors - metabolism
X Chromosome - ultrastructure
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Summary:Drosophila ISWI, a highly conserved member of the SWI2/SNF2 family of ATPases, is the catalytic subunit of three chromatin-remodeling complexes: NURF, CHRAC, and ACF. To clarify the biological functions of ISWI, we generated and characterized null and dominant-negative ISWI mutations. We found that ISWI mutations affect both cell viability and gene expression during Drosophila development. ISWI mutations also cause striking alterations in the structure of the male X chromosome. The ISWI protein does not colocalize with RNA Pol II on salivary gland polytene chromosomes, suggesting a possible role for ISWI in transcriptional repression. These findings reveal novel functions for the ISWI ATPase and underscore its importance in chromatin remodeling in vivo.
ISSN:1097-2765
1097-4164
DOI:10.1016/S1097-2765(00)80430-X