Glyceraldehyde-3-phosphate dehydrogenase gene expression in human breast cancer

• Published in: European journal of cancer (1990) 2000-05, Vol.36 (8), p.1038-1042
• Main Authors: Révillion, F, Pawlowski, V, Hornez, L, Peyrat, J.-P
• Format: Article
• Language: English
• Subjects: 5′ Nuclease assay; Adult; Aged; Aged, 80 and over; Breast Neoplasms - enzymology; Breast Neoplasms - genetics; Cell proliferation; Estradiol - metabolism; Female; Gene Expression - genetics; Gene expression normalisation; Glyceraldehyde-3-phosphate dehydrogenase (GAPDH); Glyceraldehyde-3-Phosphate Dehydrogenases - metabolism; Human breast cancer; Humans; Middle Aged; Neoplasm Proteins - metabolism; Real-time RT–PCR; Reverse Transcriptase Polymerase Chain Reaction; Reverse transcription–polymerase chain reaction (RT–PCR); RNA, Messenger - metabolism; Survival; Survival Analysis; Treatment Outcome; Tumor Cells, Cultured; Tumour aggressiveness
• ISSN: 0959-8049; 1879-0852
• DOI: 10.1016/S0959-8049(00)00051-4

Glyceraldehyde-3-phosphate dehydrogenase ( GAPDH) has been widely used as a control RNA in Northern blotting and in reverse transcriptase–polymerase chain reaction (RT–PCR) analyses. We investigated the expression of GAPDH in a large series of primary breast cancers and in MCF7 human mammary epithelial breast cancer cells treated with oestradiol. The expression of GAPDH was quantified by a real-time one-step RT–PCR assay, based upon the 5′ nuclease activity of Taq polymerase using an Abi Prism 7700 Sequence Detector System (Perkin Elmer, France). Using the Spearman test, GAPDH expression was found to correlate inversely with the age of the patients at diagnosis ( P=0.003; r=−0.147), oestradiol receptors (ER) ( P