High-abundance mRNAs in human muscle: comparison between young and old

Departments of Medicine, Pharmacology and Physiology, and Neurology, University of Rochester, Rochester, New York 14642 To gain a better understanding of the potential role of altered gene expression in the diminished muscle function in old age, we performed a broad search for transcripts expressed...

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Bibliographic Details
Published in:Journal of applied physiology (1985) 2000-07, Vol.89 (1), p.297-304
Main Authors: Welle, Stephen, Bhatt, Kirti, Thornton, Charles A
Format: Article
Language:English
Subjects:
Adult
Age Factors
Aged
Aging
Biological and medical sciences
Electron Transport Complex IV - genetics
Fundamental and applied biological sciences. Psychology
Gene Expression - physiology
Genes
Glucose - metabolism
Glycogen - metabolism
Humans
Male
Mitochondria - enzymology
Muscle Proteins - genetics
Muscle, Skeletal - metabolism
Muscular system
Myosin Heavy Chains - genetics
NADH Dehydrogenase - genetics
Proton-Translocating ATPases - genetics
Reverse Transcriptase Polymerase Chain Reaction
Ribonucleic acid
RNA
RNA, Messenger - analysis
Striated muscle. Tendons
Vertebrates: osteoarticular system, musculoskeletal system
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Summary:Departments of Medicine, Pharmacology and Physiology, and Neurology, University of Rochester, Rochester, New York 14642 To gain a better understanding of the potential role of altered gene expression in the diminished muscle function in old age, we performed a broad search for transcripts expressed at quantitatively different levels in younger (21-24 yr) and older (66-77 yr) human vastus lateralis muscle by serial analysis of gene expression (SAGE). Because SAGE was based on RNA pooled from muscle of several different subjects, relative concentrations of selected mRNAs also were determined in individual muscle samples by quantitative RT-PCR. There were 702 SAGE tags detected at least 10 times in one or both mRNA pools, and the detection frequency was different (at P  
ISSN:8750-7587
1522-1601
DOI:10.1152/jappl.2000.89.1.297