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Altered expression of natriuretic peptide receptors in proANP gene disrupted mice

The atrial natriuretic peptide (ANP) family is a complex system consisting of at least three polypeptides and at least three types of receptor. Each peptide interacts with different types of receptor at varying degrees of affinity. To determine if natriuretic peptide levels influence natriuretic pep...

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Published in:Cardiovascular research 2000-06, Vol.46 (3), p.595-603
Main Authors: VERA, Nathalie, YAT TSE, M, WATSON, John D, SARDA, Seema, STEINHELPER, Mark E, JOHN, Simon W. M, FLYNN, T. Geoffrey, PANG, Stephen C
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container_issue 3
container_start_page 595
container_title Cardiovascular research
container_volume 46
creator VERA, Nathalie
YAT TSE, M
WATSON, John D
SARDA, Seema
STEINHELPER, Mark E
JOHN, Simon W. M
FLYNN, T. Geoffrey
PANG, Stephen C
description The atrial natriuretic peptide (ANP) family is a complex system consisting of at least three polypeptides and at least three types of receptor. Each peptide interacts with different types of receptor at varying degrees of affinity. To determine if natriuretic peptide levels influence natriuretic peptide receptor expression and regulation, we examined the expression of guanylyl cyclase linked GC-A, GC-B and C-receptor in the lungs of mice with a mutation that inactivates the ANP gene (Nppa). The mRNA level of GC-A, GC-B and C-receptor in the lung were studied by ribonuclease protection assays (RPA). Results of RPA showed that although the mRNA level of GC-A and GC-B of heterozygous ANP+/- was not different from wild type ANP+/+ mice, they were significantly higher in the homozygous mutant ANP-/- mice. In addition, C-receptor mRNA level in ANP+/- and ANP-/- was significantly lower than ANP+/+ mice. The C-receptor results were confirmed by receptor binding assays and affinity cross-linking studies. Taken together these data suggest that permanent removal of ANP from the natriuretic peptide system results in an up-regulation of GC-A and GC-B, and a corresponding down-regulation of C-receptor in the lung of proANP gene disrupted mice. We postulated that changes in the natriuretic peptide receptor population may result in chronic hypertension and cardiac hypertrophy in the ANP-/- mice.
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source Oxford University Press:Jisc Collections:OUP Read and Publish 2024-2025 (2024 collection) (Reading list)
subjects Analysis of Variance
Animals
Arterial hypertension. Arterial hypotension
Atrial Natriuretic Factor - genetics
Autoradiography
Biological and medical sciences
Blood and lymphatic vessels
Cardiology. Vascular system
Experimental diseases
Gene Expression
Guanylate Cyclase - genetics
Hypertension - metabolism
Lung - metabolism
Medical sciences
Mice
Mice, Inbred C57BL
Mice, Mutant Strains
Receptors, Atrial Natriuretic Factor - genetics
Receptors, Atrial Natriuretic Factor - metabolism
RNA, Messenger - analysis
title Altered expression of natriuretic peptide receptors in proANP gene disrupted mice
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