Telomerase Activity Significantly Correlates with Chromosome Alterations, Cell Differentiation, and Proliferation in Lung Adenocarcinomas

Telomerase activity was examined by the telomeric repeat amplification protocol assay in 25 cases of lung adenocarcinoma, in relation to cancer cell differentiation, proliferation, and chromosome alterations. Telomerase activity, chromosome alterations, and cell proliferation assessed by Ki-67 label...

Full description

Saved in:
Bibliographic Details
Published in:Modern pathology 2000-07, Vol.13 (7), p.723-729
Main Authors: Fujiwara, Mutsunori, Okayasu, Isao, Takemura, Tamiko, Tanaka, Isao, Masuda, Ryo, Furuhata, Yoshiaki, Noji, Mitsuru, Oritsu, Masaru, Kato, Motonobu, Oshimura, Mitsuo
Format: Article
Language:English
Subjects:
Adenocarcinoma - enzymology
Adenocarcinoma - genetics
Adenocarcinoma - pathology
Aged
Aged, 80 and over
Cancer
Cell Differentiation
Cell Division
Cell growth
Cell proliferation
Cell Transformation, Neoplastic
Chromosome Aberrations
Chromosome alteration
Chromosomes
Clara cell type
Cytoplasm
Epithelium - enzymology
Epithelium - ultrastructure
Female
Humans
Immunoenzyme Techniques
Karyotyping
Ki-67
Ki-67 Antigen - metabolism
Labeling
Laboratory Medicine
Lung adenocarcinoma
Lung Neoplasms - enzymology
Lung Neoplasms - genetics
Lung Neoplasms - pathology
Male
Medicine
Medicine & Public Health
Microwaves
Middle Aged
original-article
Pathology
Proteins
Telomerase
Telomerase - metabolism
Thyroid gland
Tumors
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Telomerase activity was examined by the telomeric repeat amplification protocol assay in 25 cases of lung adenocarcinoma, in relation to cancer cell differentiation, proliferation, and chromosome alterations. Telomerase activity, chromosome alterations, and cell proliferation assessed by Ki-67 labeling were significantly lower (P < .001 to .05) in well-differentiated (10 cases) than in moderately differentiated (8 cases) or poorly differentiated (7 cases) lesions. Telomerase activity by semiquantitative analysis with scoring of 0 to 3 was significantly correlated with similarly graded chromosome alterations (P < .05) and Ki-67 labeling indices (P < .002). Telomerase activity and chromosome alteration (T-C) indices generated by multiplication of telomerase activity and chromosome alteration scores also showed a significant correlation with cell differentiation. The Clara cell subtype, confirmed by electron microscopic analysis, significantly predominated in the well-differentiated group, showing a low grade of telomerase activity and chromosome alterations and low Ki-67 labeling indices, suggesting clinical relevance. No significant association of telomerase activity was found with p53 protein accumulation or Bcl-2 protein expression. The good correlation of telomerase activity with chromosome alterations, cell differentiation, and Ki-67 labeling indices suggests that this parameter might have potential application in estimation of prognosis.
ISSN:0893-3952
1530-0285
DOI:10.1038/modpathol.3880125