Microchimerism in bone marrow-derived CD34+ cells of patients after liver transplantation

Lymphoid and dendritic cells of donor origin can be detected in the recipient several years after a solid organ transplantation. This phenomenon is termed microchimerism and could play a role in the induction of tolerance. The fate of other hematopoietic cells transferred by liver transplantation, i...

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Bibliographic Details
Published in:Blood 2000-07, Vol.96 (2), p.763-767
Main Authors: NIERHOFF, D, HORVATH, H. C, HOHAUS, S, MYTILINEOS, J, GOLLING, M, BUD, O, KLAR, E, OPELZ, G, VOSO, M. T, HO, A. D, HAAS, R
Format: Article
Language:English
Subjects:
Adolescent
Adult
Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy
Antigens, CD34 - analysis
Biological and medical sciences
Bone Marrow Cells - chemistry
Bone Marrow Cells - cytology
Bone Marrow Cells - immunology
Bone marrow, stem cells transplantation. Graft versus host reaction
Chimera
DNA - analysis
Female
Graft Survival
Histocompatibility
HLA-DR Antigens - genetics
HLA-DRB1 Chains
Humans
Liver Transplantation
Male
Medical sciences
Middle Aged
Polymerase Chain Reaction
Tissue Donors
Transfusions. Complications. Transfusion reactions. Cell and gene therapy
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Summary:Lymphoid and dendritic cells of donor origin can be detected in the recipient several years after a solid organ transplantation. This phenomenon is termed microchimerism and could play a role in the induction of tolerance. The fate of other hematopoietic cells transferred by liver transplantation, in particular of stem and progenitor cells, is unknown. For this reason, we studied peripheral blood and bone marrow samples of 12 patients who had received a liver transplant from an HLA-DR mismatched donor. Eight patients were long-term survivors between 2.8 and 10.1 years after allografting. CD34(+) cells from bone marrow were highly enriched with the use of a 2-step method, and a nested polymerase chain reaction was applied to detect donor cells on the basis of allelic differences of the HLA-DRB1 gene. Rigorous controls with DRB1 specificities equal to the donor and host were included. In 5 of 8 long-term liver recipients, donor-specific CD34(+) cells could be detected in bone marrow. Microchimerism in the CD34(+) cell fraction did not correlate to the chimeric status in peripheral blood. In conclusion, our results demonstrate a frequent microchimerism among bone marrow-derived CD34(+) cells after liver transplantation. The functional role of this phenomenon still needs to be defined. (Blood. 2000;96:763-767)
ISSN:0006-4971
1528-0020
DOI:10.1182/blood.V96.2.763