Decreased expression of the INK4 family of cyclin-dependent kinase inhibitors in Wilms tumor

Cyclin‐dependent kinase (CDK) inhibitors represented by the INK4 family (including p16INK4a, CDKN2A, p15INK4b, CDKN2B, p18INK4c, CDKN2C, and p19INK4d, CDKN2D) are regulators of the cell cycle shown to be aberrant in many types of human cancer. We tested the hypothesis that these CDK inhibitors are a...

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Bibliographic Details
Published in:Genes chromosomes & cancer 2000-09, Vol.29 (1), p.63-69
Main Authors: Arcellana-Panlilio, Mayi Y., Egeler, R. Maarten, Ujack, Eva, Pinto, Alfredo, Demetrick, Doug J., Robbins, Stephen M., Coppes, Max J.
Format: Article
Language:English
Subjects:
Carrier Proteins - biosynthesis
Carrier Proteins - genetics
Cell Cycle Proteins
Child
Cyclin-Dependent Kinase Inhibitor p15
Cyclin-Dependent Kinase Inhibitor p16
Cyclin-Dependent Kinases - antagonists & inhibitors
Gene Expression Regulation, Neoplastic - genetics
Genes, p16 - genetics
Genes, Tumor Suppressor - genetics
HeLa Cells
Humans
INK4a gene
Multigene Family - genetics
p16 protein
Pilot Projects
Prognosis
Tumor Suppressor Proteins
Wilms Tumor - genetics
Wilms Tumor - metabolism
Wilms' tumor
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Summary:Cyclin‐dependent kinase (CDK) inhibitors represented by the INK4 family (including p16INK4a, CDKN2A, p15INK4b, CDKN2B, p18INK4c, CDKN2C, and p19INK4d, CDKN2D) are regulators of the cell cycle shown to be aberrant in many types of human cancer. We tested the hypothesis that these CDK inhibitors are a target for altered gene expression in Wilms tumor. Using RT‐PCR, gene expression of the INK4 family was found to be decreased in 9 of 38 Wilms tumor samples obtained from the National Wilms Tumor Study Group (NWTSG) tissue bank. All the affected tumor samples were of favorable histology. Methylation‐specific PCR revealed that methylation in the p16 promoter region may be responsible for altered expression. The incidence of loss of p16 expression may increase with increasing tumor stage, i.e., 1/10 (10%) with stage I/II FH Wilms tumor, 2/10 (20%) with stage III FH Wilms tumor, and 4/10 (40%) with stage IV FH Wilms tumor. Thus, determining the expression status of the INK4 family may have potential prognostic value in the management of Wilms tumor. Genes Chromosomes Cancer 29:63–69, 2000. © 2000 Wiley‐Liss, Inc.
ISSN:1045-2257
1098-2264
DOI:10.1002/1098-2264(2000)9999:9999<::AID-GCC1006>3.0.CO;2-L