High-Performance Liquid Chromatographic Analysis of Saponin Compounds in Bupleurum falcatum

• Published in: Journal of chromatographic science 2000-06, Vol.38 (6), p.229-233
• Main Authors: Park, In-Seon, Kang, Eun Mi, Kim, Namsoo
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Chromatography, High Pressure Liquid - methods; General pharmacology; Magnoliopsida - chemistry; Medical sciences; Oleanolic Acid - analogs & derivatives; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Plant Roots - chemistry; Quality Control; Reproducibility of Results; Sapogenins - analysis; Saponins - analysis
• ISSN: 0021-9665; 1945-239X
• DOI: 10.1093/chromsci/38.6.229

A mixture of saponin compounds (saikosaponin c, a, and d) in the 70% ethanol extract of a powdered sample of Bupleuri radix are analyzed by an Inertsil ODS-3 C18 column at a flow rate of 1.0 mL/min and detection wavelength of 203 nm. Well resolved chromatograms of saikosaponin c, a, and d are obtained with a gradient elution of acetonitrile-water from 40:60 (v/v) to 50:50 (v/v). The total time required for a single analysis is approximately 20 min. Calibration curves for saikosaponin c, a, and d are linear up to 2.5 mg/mL. The coefficient of variability values for saikosaponins in the extract are below 4%, and the recoveries for saikosaponin c, a, and d are 95.2 ± 1.1, 96.5 ± 0.9, and 96.2 ± 1.0%, respectively. The changes in saikosaponin contents for a two-year growth of Bupleurum falcatum are measured by the established high-performance liquid chromatography method.