enteroaggregative Escherichia coli strain of serotype O111:H12 damages and invades cultured T84 cells and human colonic mucosa

The pathogenic mechanisms of enteroaggregative Escherichia coli (EAEC) are not well defined. We investigated the interaction of EAEC strain 236 (serotype O111:H12) with polarised Caco-2 and T84 human intestinal epithelial cells lines, and with human jejunal and colonic mucosa. Strain 236 adhered to...

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Bibliographic Details
Published in:FEMS microbiology letters 2001-09, Vol.203 (2), p.199-205
Main Authors: Abe, C.M, Knutton, S, Pedroso, M.Z, Freymuller, E, Gomes, T.A.T
Format: Article
Language:English
Subjects:
Adherence
Bacteria
Bacterial Adhesion
Bacteriology
Biological and medical sciences
Biotechnology
Caco-2 Cells
Cell Line
Cell lines
Child
Colon
Colon - microbiology
Damage
Diarrhea
E coli
Enteroaggregative Escherichia coli
Epithelial cells
Escherichia coli
Escherichia coli - classification
Escherichia coli - pathogenicity
Fundamental and applied biological sciences. Psychology
Genetics
Heterogeneity
Humans
intestinal mucosa
Intestinal Mucosa - microbiology
Intestine
Invasion
jejunum
Jejunum - microbiology
Microbiology
Microscopy, Electron
Mucosa
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
serotypes
Serotyping
Virulence
virulence factors
Virulence properties
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Summary:The pathogenic mechanisms of enteroaggregative Escherichia coli (EAEC) are not well defined. We investigated the interaction of EAEC strain 236 (serotype O111:H12) with polarised Caco-2 and T84 human intestinal epithelial cells lines, and with human jejunal and colonic mucosa. Strain 236 adhered to both polarised cell lines and to both intestinal tissue types, but caused severe damage and was invasive only in T84 cells and colonic mucosa. In contrast, prototype EAEC strain 042, which also adhered to the cultured intestinal cell lines, did not adhere to or invade jejunal or colonic tissue. These observations suggest a heterogeneity of virulence properties within the EAEC category of diarrhoea-causing E. coli.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.2001.tb10841.x