KPL1, WHICH ENCODES A NOVEL PH DOMAIN-CONTAINING PROTEIN, IS INDUCED DURING CILIATED CELL DIFFERENTIATION OF RAT TRACHEAL EPITHELIAL CELLS

Using differential display, we have identified a novel gene, KPL1, induced in rat tracheal epithelial (RTE) cells grown under conditions which stimulate ciliogenesis. The KPL1 protein is predicted to contain a pleckstrin homology (PH) domain, which has been found in numerous signal transduction and...

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Bibliographic Details
Published in:Experimental lung research 2000, Vol.26 (4), p.257-271
Main Authors: ANDREWS, K. L, POTDAR, P. D, NETTESHEIM, P, OSTROWSKI, L. E
Format: Article
Language:English
Subjects:
Air breathing
Amino Acid Sequence
Animals
Biological and medical sciences
Blood Proteins - chemistry
Cell cycle, cell proliferation
Cell Differentiation
Cell physiology
Cilia
Epithelial Cells - cytology
Fundamental and applied biological sciences. Psychology
Humans
Intracellular Signaling Peptides and Proteins
Membrane Proteins
Molecular and cellular biology
Molecular Sequence Data
Phosphoproteins - chemistry
Pleckstrin Homology Domain Tracheal Epithelial Cells Differentiation
Proteins - genetics
Rats
Rats, Inbred F344
Respiratory system: anatomy, metabolism, gas exchange, ventilatory mechanics, respiratory hemodynamics
Sequence Homology, Amino Acid
Trachea - cytology
Up-Regulation
Vertebrates: respiratory system
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Summary:Using differential display, we have identified a novel gene, KPL1, induced in rat tracheal epithelial (RTE) cells grown under conditions which stimulate ciliogenesis. The KPL1 protein is predicted to contain a pleckstrin homology (PH) domain, which has been found in numerous signal transduction and cytoskeletal proteins. These domains are thought to function by recruiting proteins to cellular membranes, and they have been shown to bind phosphoinositols and the beta/gamma subunit of G proteins. We have cloned rat and human KPL1; the predicted protein translations are 94% identical. Alternate transcripts exist in rat and human tracheal cells that predict a protein which contains a 35 amino acid insert. KPL1 was upregulated in RTE cultures undergoing mucociliary but not squamous differentiation; and in cultures undergoing mucociliary differentiation, KPL1 expression most closely paralleled that of a marker of ciliated cell differentiation (axonemal dynein heavy chain) and not a marker of mucous cell differentiation (mucin 5AC). As a new member of the family of PH domain containing proteins, KPL1may have a unique role in ciliated cell differentiation or function.
ISSN:0190-2148
1521-0499
DOI:10.1080/019021400404537