Substitution of fish species detected by thin-layer isoelectric focusing and a computer-assisted method for the evaluation of gels

Fourteen fish species susceptible to substitution were analysed by the thin-layer isoelectric focusing technique on polyacrylamide gels of pH 3.5–9.5. Four fish per species were run on the same gel to verify the possibility to differentiate them according to their protein banding patterns. The occur...

Full description

Saved in:
Bibliographic Details
Published in:Journal of Chromatography A 2000-06, Vol.880 (1), p.303-309
Main Authors: Colombo, Marco Maria, Colombo, Fabio, Biondi, Pier Antonio, Malandra, Renato, Renon, Pietro
Format: Article
Language:English
Subjects:
Animals
Fishes - classification
Image Processing, Computer-Assisted
Isoelectric Focusing - methods
Species Specificity
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Fourteen fish species susceptible to substitution were analysed by the thin-layer isoelectric focusing technique on polyacrylamide gels of pH 3.5–9.5. Four fish per species were run on the same gel to verify the possibility to differentiate them according to their protein banding patterns. The occurrence of intraspecific differences due either to electrophoretic variations or to protein polymorphism was also observed. In fact, some of them showed few dissimilarities among their protein profiles. However, the differentiation was possible for all species, even for those belonging to the same order, family and genus. Computer-based tools combined with statistical analysis were implemented and usefully applied to avoid a subjective evaluation of the isoelectric focusing gels, and to verify the reliability of the preliminary visual comparison of protein patterns.
ISSN:0021-9673
DOI:10.1016/S0021-9673(00)00331-9