Tracking pathology with proteomics: identification of in vivo degradation products of alphaB-crystallin

Soemmerring's ring is one form of "after cataract" that can occur following cataract surgery. The ring structure is formed by adherence of the anterior lens capsule to the posterior lens capsule. Epithelial cells remaining after surgery differentiate into lens fiber cells but the resu...

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Bibliographic Details
Published in:Electrophoresis 2000-06, Vol.21 (11), p.2219-2227
Main Authors: Colvis, C M, Duglas-Tabor, Y, Werth, K B, Vieira, N E, Kowalak, J A, Janjani, A, Yergey, A L, Garland, D L
Format: Article
Language:English
Subjects:
Aged
Aged, 80 and over
Amino Acid Sequence
Cataract - metabolism
Crystallins - chemistry
Crystallins - metabolism
Electrophoresis, Gel, Two-Dimensional
Humans
Middle Aged
Molecular Sequence Data
Proteome
Software
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Summary:Soemmerring's ring is one form of "after cataract" that can occur following cataract surgery. The ring structure is formed by adherence of the anterior lens capsule to the posterior lens capsule. Epithelial cells remaining after surgery differentiate into lens fiber cells but the resulting tissue mass does not remain transparent. The protein in normal lens and in Soemmerring's rings from four individuals was analyzed using two-dimensional (2-D) gel electrophoresis, matrix assisted laser desorption/ionization-time of-flight-mass spectrometry (MALDI-TOF-MS) and image analysis with Phoretix software. The 2-D protein patterns of the Soemmerring's rings were generally similar to that of cortical fiber cells of normal human lens with some notable exceptions. Several post-translationally modified forms of alphaB-crystallin(1-175) were identified. Two degradation products, alphaB-crystallin(1-170) and alphaB-crystallin(1-174), each make up 9.5-27% of the total alphaB-crystallin in the Soemmerring's rings and less than 1% in the normal lenses. Other modified forms of alphaB-crystallin are aberrant in the fiber cells of the Soemmerring rings relative to normal lens.
ISSN:0173-0835