Intensities of E. coli Nucleic Acid Raman Spectra Excited Selectively from Whole Cells with 251-nm Light

Escherichia coli bacteria in the logarithmic growth phase have been investigated by UV resonance Raman spectroscopy. Bacterial whole-cell Raman spectra excited at 251 nm reflect nearly exclusively the nucleic acid composition even though a very large fraction of the bacterial mass is composed of pro...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2000-07, Vol.72 (13), p.2981-2986
Main Authors: WU, Q., NELSON, W. H., ELLIOT, S., SPERRY, J. F., FELD, M., DASARI, R., MANOHARAN, R.
Format: Article
Language:English
Subjects:
Bacteria
Bacteriology
Biochemistry
Biological and medical sciences
Chemistry
DNA, Bacterial - chemistry
Escherichia coli - chemistry
Fundamental and applied biological sciences. Psychology
Light
Microbiology
Morphology, structure, chemical composition
Proteins
Spectrum Analysis, Raman
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Summary:Escherichia coli bacteria in the logarithmic growth phase have been investigated by UV resonance Raman spectroscopy. Bacterial whole-cell Raman spectra excited at 251 nm reflect nearly exclusively the nucleic acid composition even though a very large fraction of the bacterial mass is composed of protein. It has been demonstrated that if bacteria are grown under controlled (logarithmic growth) conditions, which give rise to organisms of known average biochemical composition, the intensities of E. coli Raman spectra can be explained quantitatively from the knowledge of component nucleic acid base resonance Raman cross sections.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac990932p