Rapid Screening of Natural Products by High-Resolution High-Temperature Gas Chromatography

The crude ethanol extracts from the leaves of three Croton hemiargyrus hemiargyreus plants are fractionated by thin-layer chromatography, yielding five fractions. The fractions and the crude extract are analyzed by high-temperature high-resolution gas chromatography coupled with mass spectrometry (H...

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Bibliographic Details
Published in:Journal of chromatographic science 2000-06, Vol.38 (6), p.234-240
Main Authors: Carbonell, Silvia Albero, de Aquino Neto, Francisco Radler, Cardoso, Jari Nobrega, dos Santos Pereira, Alberto, Amaral, Ana Claudia F., Barnes, Roderick A.
Format: Article
Language:English
Subjects:
Alcohols - analysis
Alkaloids - analysis
Biological and medical sciences
Chromatography, Gas - methods
Chromatography, Thin Layer
Ethanol
Gas Chromatography-Mass Spectrometry - methods
General pharmacology
Hot Temperature
Hydrocarbons - analysis
Medical sciences
Molecular Weight
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
Plant Extracts - chemistry
Plant Leaves - chemistry
Plants - chemistry
Terpenes - analysis
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Summary:The crude ethanol extracts from the leaves of three Croton hemiargyrus hemiargyreus plants are fractionated by thin-layer chromatography, yielding five fractions. The fractions and the crude extract are analyzed by high-temperature high-resolution gas chromatography coupled with mass spectrometry (HT-HRGC-MS). Several natural products, including thermolabile components, can be characterized directly in the samples, such as alkaloids, terpenes, flavonoids, acids, alcohols, etc. The cold on-column technique proves to be appropriate for the injection of these thermolabile compounds. HT-HRGC-MS is shown to be a valuable tool and an alternative technique to classical phytochemical procedures for the simple and fast routine analysis of natural products in crude extracts.
ISSN:0021-9665
1945-239X
DOI:10.1093/chromsci/38.6.234