Gene transfer into hepatoma cells mediated by galactose-modified α-helical peptides

• Published in: Biomaterials 2000-09, Vol.21 (17), p.1811-1819
• Main Authors: Niidome, Takuro, Urakawa, Mamiko, Sato, Haruya, Takahara, Yoshiyuki, Anai, Toyoaki, Hatakayama, Tomomitsu, Wada, Akihiro, Hirayama, Toshiya, Aoyagi, Haruhiko
• Format: Article
• Language: English
• Subjects: Amino acids; asialoglycoprotein receptors; Biological and medical sciences; Biotechnology; Carcinoma, Hepatocellular - metabolism; Carcinoma, Hepatocellular - pathology; DNA; Endocytosis; endosomes; Fundamental and applied biological sciences. Psychology; Galactose; Galactose - chemistry; Gene transfer; Genetic engineering; Genetic technics; Hepatoma; Humans; Methods. Procedures. Technologies; Peptide; Peptides - chemistry; Peptides - metabolism; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Sugars; Transfection; Tumor Cells, Cultured; Vectors (cloning, transfer, expression). Insertion sequences and transposons
• ISSN: 0142-9612; 1878-5905
• DOI: 10.1016/S0142-9612(00)00076-4

To develop a receptor-mediated gene delivery system into hepatoma cells using the cationic α-helical peptide as the gene carrier molecule, we modified an α-helical peptide, which is known to have transfection abilities into cells, with a multi-antennary ligand containing several galactose residues that provide efficient binding to the asialoglycoprotein receptor. The galactose-modified peptides formed complexes with a plasmid DNA and showed gene transfer abilities into HuH-7 cells, a human hepatoma cell line. The transfection efficiency of the peptide was increased by increasing the number of modified galactose residues on the peptide. Furthermore, considerable inhibition of the transfection efficiency by the addition of asialofetuin, which is a ligand for the asialoglycoprotein receptor, was observed in all galactose-modified peptides. Based on this result, we could confirm that the internalization of the galactose-modified peptides occurred by the receptor-mediated endocytosis pathway. In addition, to understand the transport route of the peptide–DNA complex in the cell, the effects on the transfection efficiencies with several endocytosis inhibitors were examined. As a result, it was suggested that the translocation of the peptide–DNA complex from the endocytic compartments to the cytosol mainly occurred during an early endosome step.