High galactosylation of oligosaccharides in umbilical cord blood IgG, and its relationship to placental function

• Published in: Clinica chimica acta 2000-09, Vol.299 (1), p.169-177
• Main Authors: Kimura, Satoshi, Numaguchi, Masahide, Kaizu, Tokio, Kim, Donghyun, Takagi, Yasushi, Gomi, Kunihide
• Format: Article
• Language: English
• Subjects: Adult; Analysis of the immune response. Humoral and cellular immunity; Biological and medical sciences; Carbohydrate; Chromatography, High Pressure Liquid; Cord blood; Diseases of mother, fetus and pregnancy; Female; Fetal Blood - chemistry; Fluorenes; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Galactose - metabolism; Galactosylation; Gynecology. Andrology. Obstetrics; Humans; IgG; Immunobiology; Immunoglobulin G - chemistry; Indicators and Reagents; Infant, Newborn; Male; Medical sciences; Miscellaneous; Oligosaccharides - blood; Placenta - chemistry; Placenta - physiology; Placental function; Pregnancy. Fetus. Placenta; Regulatory factors and their cellular receptors
• ISSN: 0009-8981; 1873-3492
• DOI: 10.1016/S0009-8981(00)00289-8

N-linked oligosaccharides on human serum IgGs have been reported to modulate IgG function. We studied umbilical cord blood to determine whether neonatal IgGs have characteristic structures related to developmental and pathological status. Oligosaccharide patterns of serum IgG from 45 umbilical cord blood samples were characterized by HPLC, and compared with those of serum IgG from 11 normal adults. Oligosaccharyl amines from purified IgG were released by recombinant N-glycanase, labeled with fluorescence reagent FMOC (9-fluorenylmethyl chloroformate), and analyzed quantitatively by high-pressure liquid chromatography (HPLC). Increased galactosylation was observed in cord blood. The ratio of galactosylated to non-galactosylated oligosaccharides on IgG was 7.90±3.92 (mean±S.D.) in cord blood, significantly higher than the ratio in adults (1.60±0.62, P