Identification of a histamine H(3)-like receptor in the zebrafish (Danio rerio) brain

The distribution of histaminergic fibers in the zebrafish brain was recently shown to resemble that in mammals. Expression of L-histidine decarboxylase (HDC) mRNA was shown only in the area corresponding to that expressing HDC in mammals. This indicates that the zebrafish could be a useful model for...

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Bibliographic Details
Published in:Journal of neurochemistry 2000-08, Vol.75 (2), p.718-724
Main Authors: Peitsaro, N, Anichtchik, O V, Panula, P
Format: Article
Language:English
Subjects:
Animals
Autoradiography
Brain - cytology
Brain - metabolism
Cimetidine - pharmacology
Female
Guanosine 5'-O-(3-Thiotriphosphate) - pharmacokinetics
Histamine Agonists - pharmacokinetics
Histamine Antagonists - pharmacology
Imidazoles - pharmacology
Kinetics
Male
Methylhistamines - pharmacokinetics
Piperidines - pharmacology
Pyrilamine - pharmacology
Radioligand Assay
Receptors, Histamine H3 - analysis
Receptors, Histamine H3 - metabolism
Sulfur Radioisotopes
Thiourea - analogs & derivatives
Thiourea - pharmacology
Tritium
Zebrafish
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Summary:The distribution of histaminergic fibers in the zebrafish brain was recently shown to resemble that in mammals. Expression of L-histidine decarboxylase (HDC) mRNA was shown only in the area corresponding to that expressing HDC in mammals. This indicates that the zebrafish could be a useful model for studies on the function of the brain histaminergic system. In this study an H(3)-like receptor is identified in zebrafish brain. With binding studies using N-alpha-[(3)H]methylhistamine on zebrafish brain sections, signals were observed in several regions. Highest densities were detected in optic tectum and hypothalamus. The autoradiographic signal was abolished completely by the H(3)-specific antagonist clobenpropit and significantly reduced by another H(3) antagonist, thioperamide. Histamine and immepip induced an increase of guanosine 5'-(gamma-[(35)S]thio)triphosphate binding in several areas of the zebrafish brain. The activation was blocked with clobenpropit but not with cimetidine or mepyramine. These results indicate that the zebrafish has a histamine H(3)-like receptor that functionally interacts with the inhibitory, G(i)/G(o), class of G proteins. No previous evidence for a histamine receptor in zebrafish exists. The receptor described here is apparently similar to the mammalian H(3) receptor, making this the first description of a histamine H(3)-like receptor in a lower vertebrate.
ISSN:0022-3042