Determination of the major mercapturic acids of 1,3-butadiene in human and rat urine using liquid chromatography with tandem mass spectrometry

The major urinary metabolites of 1,3-butadiene are monohydroxybutenyl-mercapturic acids (MHBMA) and dihydroxy-butyl-mercapturic acid (DHBMA). These metabolites can be used as biomarkers of exposure to this diene. In order to determine the smoking-related exposure to 1,3-butadiene, we have developed...

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Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2003-10, Vol.796 (1), p.131-140
Main Authors: Urban, Michael, Gilch, Gerhard, Schepers, Georg, Miert, Erik van, Scherer, Gerhard
Format: Article
Language:English
Subjects:
1,3-Butadiene
Acetylcysteine - urine
Adult
Animals
Biological and medical sciences
Calibration
Chemical and industrial products toxicology. Toxic occupational diseases
Chromatography, High Pressure Liquid - methods
Gas, fumes
Humans
Male
Mass Spectrometry - methods
Medical sciences
Mercapturic acids
Rats
Rats, Sprague-Dawley
Reproducibility of Results
Sensitivity and Specificity
Toxicology
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cited_by cdi_FETCH-LOGICAL-c391t-9f4c77e9e4eed03536a9300e10567de5f38ca8f16c165b8970119a09971df72a3
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creator Urban, Michael
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description The major urinary metabolites of 1,3-butadiene are monohydroxybutenyl-mercapturic acids (MHBMA) and dihydroxy-butyl-mercapturic acid (DHBMA). These metabolites can be used as biomarkers of exposure to this diene. In order to determine the smoking-related exposure to 1,3-butadiene, we have developed a rapid LC-MS/MS method for the determination of MHBMA and DHBMA in urine of humans and rats. The method requires 2–5 ml of urine which is solid phase extracted prior to LC-MS/MS analysis. Precision for MHBMA is ≤11.2% for human and ≤17% for rat urine. Corresponding values for DHBMA are ≤7.2 and ≤19%, respectively. Recovery rates are approximately 100% for both analytes in human urine and about 115% in rat urine. Limits of detection (LOD) are for humans 0.9 and 23 ng/ml and for rats 1.5 and 33 ng/ml for MHBMA and DHBMA, respectively. Application of the method to urine of humans and rats showed a significant effect of tobacco smoke exposure on the urinary excretion of MHBMA and the metabolic ratio DHBMA/(DHBMA + MHBMA).
doi_str_mv 10.1016/j.jchromb.2003.08.009
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subjects 1,3-Butadiene
Acetylcysteine - urine
Adult
Animals
Biological and medical sciences
Calibration
Chemical and industrial products toxicology. Toxic occupational diseases
Chromatography, High Pressure Liquid - methods
Gas, fumes
Humans
Male
Mass Spectrometry - methods
Medical sciences
Mercapturic acids
Rats
Rats, Sprague-Dawley
Reproducibility of Results
Sensitivity and Specificity
Toxicology
title Determination of the major mercapturic acids of 1,3-butadiene in human and rat urine using liquid chromatography with tandem mass spectrometry
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