Macrophage-osteoclast differentiation and bone resorption in osteoarthrotic subchondral acetabular cysts

A macrophage infiltrate is commonly found in enlarging subchondral cysts in osteoarthrosis (OA) and the surrounding bone. To determine whether osteoclast differentiation by these cells contributes to the increase in the number of osteoclasts and bone resorption that accompanies OA cyst enlargement,...

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Bibliographic Details
Published in:Acta orthopaedica 2000, Vol.71 (3), p.255-261
Main Authors: Sabokbar, Afsie, Crawford, Ross, Murray, David W, Athanasou, Nicholas A
Format: Article
Language:English
Subjects:
Acetabulum - pathology
Acid Phosphatase - analysis
Aged
Biological and medical sciences
Bone Cysts - complications
Bone Cysts - metabolism
Bone Cysts - pathology
Bone Resorption - complications
Bone Resorption - pathology
Calcitriol - pharmacology
Cell Differentiation
Coculture Techniques
Diseases of the osteoarticular system
Female
Femur Head - pathology
Humans
Isoenzymes - analysis
Macrophage Colony-Stimulating Factor - pharmacology
Macrophage-1 Antigen - analysis
Macrophages - chemistry
Macrophages - cytology
Macrophages - pathology
Male
Medical sciences
Middle Aged
Osteoarthritis
Osteoarthritis - complications
Osteoarthritis - pathology
Osteoclasts - chemistry
Osteoclasts - cytology
Osteoclasts - pathology
Receptors, Vitronectin - analysis
Tartrate-Resistant Acid Phosphatase
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Summary:A macrophage infiltrate is commonly found in enlarging subchondral cysts in osteoarthrosis (OA) and the surrounding bone. To determine whether osteoclast differentiation by these cells contributes to the increase in the number of osteoclasts and bone resorption that accompanies OA cyst enlargement, we isolated macrophages from the wall of OA cysts and co-cultured them with osteoblast-like UMR106 cells in the presence or absence of 1,25(OH) 2 D 3 and M-CSF. After 14 days of incubation, co-cultures of UMR106 cells and cyst-derived macrophages showed evidence of osteoclast differentiation by expression of TRAP, VNR and formation of numerous lacunar pits. We found that, unlike osteoclast precursors in monocyte and other tissue macrophage populations, the addition of M-CSF to medium is not required for osteoclast differentiation. Our findings suggest that macrophage-osteoclast differentiation is one means whereby the osteolysis associated with the enlargement of OA cysts could be effected.
ISSN:1745-3674
0001-6470
1745-3682
DOI:10.1080/000164700317411843