Inhibition of MMP-1 expression by antisense RNA decreases invasiveness of human chondrosarcoma

We previously reported that an elevated level of matrix metalloproteinase-1 (MMP-1) gene expression in patients with chondrosarcoma has a strong statistical correlation with recurrence and in vitro invasion. In the present study, we used an antisense RNA strategy for MMP-1 inhibition to determine if...

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Bibliographic Details
Published in:Journal of orthopaedic research 2003-11, Vol.21 (6), p.1063-1070
Main Authors: Jiang, Xiaoling, Dutton, Charyl M, Qi, Wen-ning, Block, Joel A, Brodt, Pnina, Durko, Margaret, Scully, Sean P
Format: Article
Language:English
Subjects:
Antisense RNA
Cell Movement - genetics
Chondrosarcoma
Chondrosarcoma - enzymology
Chondrosarcoma - genetics
Chondrosarcoma - pathology
Collagenases - metabolism
Down-Regulation
Humans
Invasion
Matrix Metalloproteinase 1 - genetics
Matrix Metalloproteinase 1 - metabolism
Matrix Metalloproteinase Inhibitors
Metastasis
MMP-1
Neoplasm Invasiveness - genetics
Neoplasm Invasiveness - pathology
Reverse Transcriptase Polymerase Chain Reaction
RNA, Antisense
RNA, Messenger - genetics
Transfection
Tumor Cells, Cultured - drug effects
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Summary:We previously reported that an elevated level of matrix metalloproteinase-1 (MMP-1) gene expression in patients with chondrosarcoma has a strong statistical correlation with recurrence and in vitro invasion. In the present study, we used an antisense RNA strategy for MMP-1 inhibition to determine if this would affect the invasive characteristics of the cells. We transfected a human chondrosarcoma cell line with a retroviral plasmid expressing a 770 bp genomic fragment of the human MMP-1 gene in the sense or antisense orientation. The results show that cells transfected with the MMP-1 antisense fragment had a significant decrease in both MMP-1 protein and enzyme activity ( p
ISSN:0736-0266
1554-527X
DOI:10.1016/S0736-0266(03)00079-2