Developmentally and transgene regulated nuclear processing of primary transcripts of chalcone synthase A in petunia

Summary The introduction of chalcone synthase A transgenes into petunia plants can result in degradation of chalcone synthase A RNAs and loss of chalcone synthase, a process called cosuppression or post‐transcriptional gene silencing. Here we show that the RNA degradation is associated with changes...

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Bibliographic Details
Published in:The Plant journal : for cell and molecular biology 2000-07, Vol.23 (1), p.63-72
Main Authors: Metzlaff, Michael, O'dell, Michael, Hellens, Roger, Flavell, Richard B.
Format: Article
Language:English
Subjects:
Acyltransferases - genetics
Biological and medical sciences
Biotechnology
Cell Nucleus - enzymology
chalcone synthase A
co‐suppression
ds RNA
Fundamental and applied biological sciences. Psychology
Gene expression
Gene Expression Regulation
gene regulation
Genetic engineering
Genetic technics
Methods. Procedures. Technologies
Molecular and cellular biology
Molecular genetics
Nucleic Acid Hybridization
Petunia
Plants, Genetically Modified - enzymology
Plants, Genetically Modified - genetics
premRNA processing
Reverse Transcriptase Polymerase Chain Reaction
RNA Splicing
RNA, Messenger - genetics
Solanaceae - enzymology
Solanaceae - genetics
Transgenes
Transgenic animals and transgenic plants
Transgenic plants
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Summary:Summary The introduction of chalcone synthase A transgenes into petunia plants can result in degradation of chalcone synthase A RNAs and loss of chalcone synthase, a process called cosuppression or post‐transcriptional gene silencing. Here we show that the RNA degradation is associated with changes in premRNA processing, i.e. loss of tissue specificity in transcript cleavage patterns, accumulation of unspliced molecules, and use of template‐specific secondary poly(A) sites. These changes can also be observed at a lower level in leaves but not flowers of nontransgenic petunias. Based on this, a model is presented of how transgenes may disturb the carefully evolved, developmentally controlled post‐transcriptional regulation of chalcone synthase gene expression by influencing the survival rate of the endogenous and their own mRNA.
ISSN:0960-7412
1365-313X
DOI:10.1046/j.1365-313x.2000.00793.x