Expression of lysophosphatidic acid receptor in rat astrocytes : Mitogenic effect and expression of neurotrophic genes

Lysophosphatidic acid (LPA) is a phospholipid mediator with a variety of biological activities. It remains unknown, however, which cells in the brain express the LPA receptor. The present study was undertaken to identify cells in the rat brain expressing functional LPA receptors, and to explore biol...

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Bibliographic Details
Published in:Neurochemical research 2000-05, Vol.25 (5), p.573-582
Main Authors: TABUCHI, S, KUME, K, AIHARA, M, SHIMIZU, T
Format: Article
Language:English
Subjects:
3T3 Cells
Animals
Astrocytes - cytology
Astrocytes - drug effects
Astrocytes - physiology
Biochemistry and metabolism
Biological and medical sciences
Brain - cytology
Brain - physiology
Calcium - metabolism
Cell Division - drug effects
Cells, Cultured
Central nervous system
Cyclooxygenase 2
Embryo, Mammalian
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation - drug effects
Genes, fos - drug effects
gliosis
immediate early genes
Isoenzymes - genetics
lysophosphatidic acid receptors
Lysophospholipids - pharmacology
Mice
Mice, Inbred ICR
PC12 Cells
Prostaglandin-Endoperoxide Synthases - genetics
Rats
Rats, Wistar
Receptors, Cell Surface - physiology
Receptors, G-Protein-Coupled
Receptors, Lysophosphatidic Acid
Transcription, Genetic - drug effects
Vertebrates: nervous system and sense organs
Citations: Items that cite this one
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Summary:Lysophosphatidic acid (LPA) is a phospholipid mediator with a variety of biological activities. It remains unknown, however, which cells in the brain express the LPA receptor. The present study was undertaken to identify cells in the rat brain expressing functional LPA receptors, and to explore biological roles of LPA in these cells. We found that the LPA receptor was most dominantly expressed in rat astrocytes, determined by LPA-induced Ca2+ imaging, and by Northern blot analyses. LPA induced a mitogenic response and expression of immediate early genes in astrocytes, through pertussis-toxin sensitive G-protein(s). LPA also stimulated the expression of various cytokine genes, including nerve growth factor, interleukin (IL)-1beta, IL-3 and IL-6. Thus, astrocytes are the major target of LPA in the brain. We propose that LPA may play important roles in neuronal development, gliosis and wound-healing process in the brain.
ISSN:0364-3190
1573-6903
DOI:10.1023/a:1007542532395