Deoxyribonuclease activity in Selenomonas ruminantium, streptococcus bovis, and Bacteroides ovatus

Six Selenomonas ruminantium strains (132c, JW13, SRK1, 179f, 5521c1, and 5934e), Streptococcus bovis JB1, and Bacteroides ovatus V975 were examined for nuclease activity as well as the ability to utilize nucleic acids, ribose, and 2-deoxyribose. Nuclease activity was detected in sonicated cells and...

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Bibliographic Details
Published in:Current microbiology 2000-09, Vol.41 (3), p.182-186
Main Authors: AL-KHALDI, Sufian F, DUROCHER, Lawren L, MARTIN, Scott A
Format: Article
Language:English
Subjects:
Acetic acid
Animals
Bacteriology
Bacteroides - enzymology
Bacteroides - growth & development
Bacteroides ovatus
Biological and medical sciences
Deoxyribonucleases - metabolism
Deoxyribonucleic acid
Deoxyribonucleosides - metabolism
Deoxyribose - metabolism
DNA
Fundamental and applied biological sciences. Psychology
Metabolism. Enzymes
Microbiology
Nucleic acids
Nucleic Acids - metabolism
Protease inhibitors
Ribose - metabolism
Ruminants
Selenomonas - enzymology
Selenomonas - growth & development
Streptococcus bovis - enzymology
Streptococcus bovis - growth & development
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Summary:Six Selenomonas ruminantium strains (132c, JW13, SRK1, 179f, 5521c1, and 5934e), Streptococcus bovis JB1, and Bacteroides ovatus V975 were examined for nuclease activity as well as the ability to utilize nucleic acids, ribose, and 2-deoxyribose. Nuclease activity was detected in sonicated cells and culture supernatants for all bacteria except S. ruminantium JW13 and 179f sonicated cells. S. ruminantium strains were able to utilize several deoxyribonucleosides, while S. bovis JB1 and B. ovatus V975 showed little or no growth on all deoxyribonucleosides. When S. ruminantium strains 5934e, 132c, JW13, and SRK1 were incubated in medium that contained 15 mm ribose, the major end products were acetate, propionate, and lactate. S. ruminantium 5521c1 and S. bovis JB1 did not grow on ribose, and none of the S. ruminantium strains or S. bovis JB1 grew on 15 mm 2-deoxyribose. In contrast, B. ovatus V975 was able to grow on ribose and 2-deoxyribose. In conclusion, all S. ruminantium strains, S. bovis JB1, and B. ovatus V975 had nuclease activity. However, not all bacteria were able to utilize deoxyribonucleosides, ribose, or 2-deoxyribose.
ISSN:0343-8651
1432-0991
DOI:10.1007/s002840010115