Molecular cloning and characterization of a putative cyclic nucleotide-gated channel from Drosophila melanogaster

We have cloned a cDNA encoding a putative cyclic nucleotide‐gated (CNG) channel from Drosophila melanogaster. The N‐terminal half of the predicted protein, designated as CNGL, shows a high degree of sequence similarity with the known CNG channel proteins. CNGL has a long hydrophilic C‐terminal stret...

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Bibliographic Details
Published in:Insect molecular biology 2000-06, Vol.9 (3), p.283-292
Main Authors: Miyazu, M., Tanimura, T., Sokabe, M.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
antennal glomeruli
Base Sequence
Calcium Channels - genetics
Chromosomes
cloning
Cloning, Molecular
Cyclic Nucleotide-Gated Cation Channels
cyclic nucleotide-gated channel
cyclic nucleotides
DNA, Complementary
Drosophila
Drosophila melanogaster
Drosophila melanogaster - genetics
Drosophila Proteins
Gene Expression
Genes, Insect
Immunoenzyme Techniques
immunohistochemistry
In Situ Hybridization - methods
medulla
Molecular Sequence Data
Rabbits
RNA, Messenger
Sequence Homology, Amino Acid
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Summary:We have cloned a cDNA encoding a putative cyclic nucleotide‐gated (CNG) channel from Drosophila melanogaster. The N‐terminal half of the predicted protein, designated as CNGL, shows a high degree of sequence similarity with the known CNG channel proteins. CNGL has a long hydrophilic C‐terminal stretch that is absent in other CNG channels. Northern blot analysis revealed that the messenger RNA (mRNA) corresponding to the size of the cloned cDNA is expressed in Drosophila heads. Immunolocalization studies showed that CNGL is expressed in the brain, including the medulla, lobulla and lobulla plate, the antennal lobe glomeruli, and mushroom bodies. These results suggest a possible role of the putative CNGL channel in the processing of visual and olfactory information in the nervous system of Drosophila.
ISSN:0962-1075
1365-2583
DOI:10.1046/j.1365-2583.2000.00186.x