Nucleotide sequence of α-galactosidase MEL gene from Zygosaccharomyces mrakii

The region encompassing the alpha-galactosidase MELr gene was amplified from Zygosaccharomyces mrakii IFO 1835(T) by inverse-PCR and then sequenced. The nucleotide sequence of this region revealed a single open reading frame of 1410 bp encoding a 470 amino acid protein with a molecular weight of 51,...

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Bibliographic Details
Published in:Current microbiology 2000-09, Vol.41 (3), p.220-222
Main Authors: ODA, Yuji, FUJISAWA, Tomoko
Format: Article
Language:English
Subjects:
alpha-Galactosidase - chemistry
alpha-Galactosidase - genetics
Amino acid sequence
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
Fundamental and applied biological sciences. Psychology
Fungal Proteins - chemistry
Fungal Proteins - genetics
Genes, Fungal
Genetics
Growth, nutrition, metabolism, transports, enzymes. Molecular biology
Microbiology
Mission oriented research
Molecular Sequence Data
Molecular Weight
Mycology
Saccharomyces
Zygosaccharomyces - enzymology
Zygosaccharomyces - genetics
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Summary:The region encompassing the alpha-galactosidase MELr gene was amplified from Zygosaccharomyces mrakii IFO 1835(T) by inverse-PCR and then sequenced. The nucleotide sequence of this region revealed a single open reading frame of 1410 bp encoding a 470 amino acid protein with a molecular weight of 51,909. The similarity of the deduced mature protein to other yeast alpha-galactosidases was 63.3% to Zygosaccharomyces cidri, 71.5% to Torulaspora delbrueckii, and 70. 7-73.9% to Saccharomyces species. The nucleotide and amino acid sequences are deposited in the DDBJ/EMBL/GenBank database under Accession Number AB030209.
ISSN:0343-8651
1432-0991
DOI:10.1007/s002840010123