Soluble Adenylyl Cyclase as an Evolutionarily Conserved Bicarbonate Sensor

Spermatozoa undergo a poorly understood activation process induced by bicarbonate and mediated by cyclic adenosine 3′,5′-monophosphate (cAMP). It has been assumed that bicarbonate mediates its effects through changes in intracellular pH or membrane potential; however, we demonstrate here that bicarb...

Full description

Saved in:
Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 2000-07, Vol.289 (5479), p.625-628
Main Authors: Chen, Yanqiu, Cann, Martin J., Litvin, Tatiana N., Iourgenko, Vadim, Sinclair, Meeghan L., Levin, Lonny R., Buck, Jochen
Format: Article
Language:English
Subjects:
adenylyl cyclase
Adenylyl Cyclases - chemistry
Adenylyl Cyclases - genetics
Adenylyl Cyclases - isolation & purification
Adenylyl Cyclases - metabolism
Animals
bicarbonate
Bicarbonates
Bicarbonates - metabolism
Bicarbonates - pharmacology
Biological and medical sciences
Catalytic Domain
Cell Line
Cell lines
Cell physiology
Cyanobacteria
Cyanobacteria - enzymology
Cyclic AMP - metabolism
Cytosol
Enzyme Activation
Error rates
Evolution, Molecular
Fundamental and applied biological sciences. Psychology
HEK293 cells
Humans
Hydrogen-Ion Concentration
Kidneys
Male
Molecular and cellular biology
Phylogeny
Physiological regulation
Rats
Recombinant Proteins - isolation & purification
Recombinant Proteins - metabolism
Second Messenger Systems
Signal Transduction
Solubility
Sperm
Sperm Capacitation
Spermatozoa
Spermatozoa - enzymology
Spermatozoa - metabolism
Spermatozoa - physiology
Testes
Testis - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Spermatozoa undergo a poorly understood activation process induced by bicarbonate and mediated by cyclic adenosine 3′,5′-monophosphate (cAMP). It has been assumed that bicarbonate mediates its effects through changes in intracellular pH or membrane potential; however, we demonstrate here that bicarbonate directly stimulates mammalian soluble adenylyl cyclase (sAC) activity in vivo and in vitro in a pH-independent manner. sAC is most similar to adenylyl cyclases from cyanobacteria, and bicarbonate regulation of cyclase activity is conserved in these early forms of life. sAC is also expressed in other bicarbonate-responsive tissues, which suggests that bicarbonate regulation of cAMP signaling plays a fundamental role in many biological systems.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.289.5479.625