Rapid Antibody-Based Field Test To Distinguish Between Helicoverpa armigera (Lepidoptera: Noctuidae) and Helicoverpa punctigera (Lepidoptera: Noctuidae)

Helicoverpa armigera (Hübner) and Helicoverpa punctigera (Wallengren) are the two most important insect pests of cotton production in Australia and require application of insecticides to control them. H. armigera has developed resistance to several insecticides but H. punctigera has not. Cost-effect...

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Bibliographic Details
Published in:Journal of economic entomology 2000-06, Vol.93 (3), p.878-891
Main Authors: Trowell, Stephen C., Forrester, Neil W., Garsia, Kim A., Lang, Gaby A., Bird, Lisa J., Hill, Amanda S., Skerritt, John H., Daly, Joanne C.
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal - immunology
antibody
Antigens - immunology
Antigens - metabolism
Biological and medical sciences
Carrier Proteins - immunology
Chemical control
Control
diagnostic test
enzyme-linked immunosorbent assay
Fundamental and applied biological sciences. Psychology
Helicoverpa armigera
Helicoverpa punctigera
Hemolymph - immunology
insecticide resistance
INSECTICIDE RESISTANCE AND INSECTICIDE MANAGEMENT
Lipoproteins - immunology
Mice
Moths - classification
Moths - immunology
Ovum - immunology
Phytopathology. Animal pests. Plant and forest protection
Protozoa. Invertebrates
Time Factors
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Summary:Helicoverpa armigera (Hübner) and Helicoverpa punctigera (Wallengren) are the two most important insect pests of cotton production in Australia and require application of insecticides to control them. H. armigera has developed resistance to several insecticides but H. punctigera has not. Cost-effective management of insecticide resistance requires that growers be able to determine the proportion of H. armigera eggs or young larvae present on their crop before applying insecticides. This is impossible visually. We generated two monoclonal antibodies that reacted with the insect protein “lipophorin” and were capable of discriminating individuals of the two species at all life-stages. The antibodies were incorporated into a rapid test kit that was tested under field conditions over two growing seasons. Results obtained with the kit agreed closely with those obtained by rearing larvae through to second instar.
ISSN:0022-0493
1938-291X
0022-0493
DOI:10.1603/0022-0493-93.3.878