Oxygen consumption and chloride secretion in rat distal colon isolated mucosa

The aerobic metabolic cost of chloride secretion was studied in rat distal colon isolated mucosa under several conditions by simultaneous measurement of short-circuit current and oxygen consumption under conditions that preserve vectorial ion transport. A low-chloride solution and the presence of bu...

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Bibliographic Details
Published in:Digestive diseases and sciences 2003-09, Vol.48 (9), p.1767-1773
Main Authors: SARAVI, Fernando D, SALDENA, Teobaldo A, CARRERA, Cristian A, IBANEZ, Jorge E, CINCUNEGUI, Liliana M, CARRA, Graciela E
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Chlorides - metabolism
Colon - metabolism
Fundamental and applied biological sciences. Psychology
In Vitro Techniques
Intestinal Mucosa - metabolism
Intestine. Mesentery
Male
Ouabain - pharmacology
Oxygen Consumption - physiology
Rats
Rats, Wistar
Serotonin - pharmacology
Tropical medicine
Vertebrates: digestive system
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Summary:The aerobic metabolic cost of chloride secretion was studied in rat distal colon isolated mucosa under several conditions by simultaneous measurement of short-circuit current and oxygen consumption under conditions that preserve vectorial ion transport. A low-chloride solution and the presence of bumetanide plus diphenylamine-2-carboxylate reduced short-circuit current by 75% and oxygen consumption by 25%. Ouabain decreased short-circuit current by 93% and oxygen consumption by 32%. Serotonin increased both variables by 59% and 33%, respectively. Bumetanide and diphenylamine-2-carboxylate reduced but did not abolish the effect of serotonin on short-circuit current and oxygen consumption. Changes in short-circuit current and oxygen consumption were linearly correlated under all conditions tested. It is concluded that, in the unstimulated rat distal colon epithelium, chloride secretion accounts for about 75% of ouabain-sensitive short-circuit current and oxygen consumption. Stimulated chloride secretion may demand over 40% of total oxygen consumption.
ISSN:0163-2116
1573-2568
DOI:10.1023/A:1025451213866