sICAM-1 correlates with myocardial ICAM-1 expression in dilated cardiomyopathy

• Published in: International journal of cardiology 2003-10, Vol.91 (2), p.153-161
• Main Authors: Noutsias, Michel, Hohmann, Claudia, Pauschinger, Matthias, Schwimmbeck, Peter-Lothar, Ostermann, Karsten, Rode, Ullrich, Yacoub, Magdi H, Kühl, Uwe, Schultheiss, Heinz-Peter
• Format: Article
• Language: English
• Subjects: Adult; Antigens, Differentiation, T-Lymphocyte - metabolism; Biological and medical sciences; Biopsy; Cardiology. Vascular system; Cardiomyopathy, Dilated - metabolism; Cardiomyopathy, Dilated - physiopathology; Digital image analysis; Dilated cardiomyopathy; ELISA; Endothelium, Vascular - metabolism; Endothelium, Vascular - pathology; Endothelium, Vascular - physiopathology; False Positive Reactions; Female; Heart; Humans; ICAM-1; Immune system; Immunohistochemistry; Inflammatory cardiomyopathy; Intercellular Adhesion Molecule-1 - biosynthesis; Male; Medical sciences; Middle Aged; Multivariate Analysis; Myocarditis. Cardiomyopathies; Myocardium - metabolism; Myocardium - pathology; Predictive Value of Tests; Sensitivity and Specificity; sICAM-1; Solubility; Statistics as Topic; Stroke Volume - physiology; Tissue Donors
• ISSN: 0167-5273; 1874-1754
• DOI: 10.1016/S0167-5273(03)00033-0

Background: Dilated cardiomyopathy (DCM) is etiopathogenically linked to intramyocardial inflammation, which is reflected by ICAM-1 abundance. We investigated whether soluble ICAM-1 (sICAM-1) levels in the sera of DCM patients are associated with intramyocardial ICAM-1 expression. Methods: Immunohistochemically detected ICAM-1 expression was quantified semiquantitatively in endomyocardial biopsies from DCM patients ( n=45; n=17 females; age: 48±15 years) and from n=12 donor hearts (controls) by a human observer (baseline vs. enhanced expression) and quantitatively by a digital image analysis (DIA) system. The DIA-measured qualities were area fraction (AF), surface–volume ratio (SVR) and integral optical density (ID). The sICAM-1 levels of the DCM patients and n=12 healthy volunteers (controls) were measured by ELISA (means of duplicate measurements). Intramyocardial ICAM-1 expression and sICAM-1 levels were compared in these DCM patients. Results: Of the DCM patients, n=24 (53%) demonstrated statistically higher sICAM-1 levels compared to controls (>198 ng/ml). By semiquantitative and quantitative DIA evaluation, endothelial ICAM-1 abundance was present in n=25 (56%) of the DCM biopsies. sICAM-1 correlated significantly ( P